View source for 4nnc


==Ternary complex of ObcA with C4-CoA adduct and oxalate==
<StructureSection load='4nnc' size='340' side='right'caption='[[4nnc]], [[Resolution|resolution]] 2.28&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4nnc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Burkholderia_glumae_BGR1 Burkholderia glumae BGR1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NNC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NNC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.279&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2KQ:(3S)-3-[2-[3-[[(2R)-4-[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-3-oxidanyl-butanoic+acid'>2KQ</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=OXL:OXALATE+ION'>OXL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nnc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nnc OCA], [https://pdbe.org/4nnc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nnc RCSB], [https://www.ebi.ac.uk/pdbsum/4nnc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nnc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C5AJX5_BURGB C5AJX5_BURGB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Burkholderia species utilize acetyl-CoA and oxaloacetate, substrates for citrate synthase in the TCA cycle, to produce oxalic acid in response to bacterial cell to cell communication, called quorum sensing. Quorum sensing-mediated oxalogenesis via a sequential reaction by ObcA and ObcB counteracts the population-collapsing alkaline pH of the stationary growth phase. Thus, the oxalic acid produced plays an essential role as an excreted public good for survival of the group. Here, we report structural and functional analyses of ObcA, revealing mechanistic features distinct from those of citrate synthase. ObcA exhibits a unique fold, in which a (beta/alpha)8-barrel fold is located in the C-domain with the N-domain inserted into a loop following alpha1 in the barrel fold. Structural analyses of the complexes with oxaloacetate and with a bisubstrate adduct indicate that each of the oxaloacetate and acetyl-CoA substrates is bound to an independent site near the metal coordination shell in the barrel fold. In catalysis, oxaloacetate serves as a nucleophile by forming an enolate intermediate mediated by Tyr(322) as a general base, which then attacks the thioester carbonyl carbon of acetyl-CoA to yield a tetrahedral adduct between the two substrates. Therefore, ObcA catalyzes its reaction by combining the enolase and acetyltransferase superfamilies, but the presence of the metal coordination shell and the absence of general acid(s) produces an unusual tetrahedral CoA adduct as a stable product. These results provide the structural basis for understanding the first step in oxalogenesis and constitute an example of the functional diversity of an enzyme for survival and adaptation in the environment.

Structural Basis for Bacterial Quorum Sensing-mediated Oxalogenesis.,Oh J, Goo E, Hwang I, Rhee S J Biol Chem. 2014 Apr 18;289(16):11465-75. doi: 10.1074/jbc.M113.543462. Epub, 2014 Mar 10. PMID:24616091<ref>PMID:24616091</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4nnc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Burkholderia glumae BGR1]]
[[Category: Large Structures]]
[[Category: Goo E]]
[[Category: Hwang I]]
[[Category: Oh JT]]
[[Category: Rhee S]]