View source for 4nwn


==Computationally Designed Two-Component Self-Assembling Tetrahedral Cage T32-28==
<StructureSection load='4nwn' size='340' side='right'caption='[[4nwn]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4nwn]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_subsp._jejuni_81-176 Campylobacter jejuni subsp. jejuni 81-176] and [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium_str._STm5 Salmonella enterica subsp. enterica serovar Typhimurium str. STm5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NWN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NWN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nwn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nwn OCA], [https://pdbe.org/4nwn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nwn RCSB], [https://www.ebi.ac.uk/pdbsum/4nwn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nwn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0H3PA01_CAMJJ A0A0H3PA01_CAMJJ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The self-assembly of proteins into highly ordered nanoscale architectures is a hallmark of biological systems. The sophisticated functions of these molecular machines have inspired the development of methods to engineer self-assembling protein nanostructures; however, the design of multi-component protein nanomaterials with high accuracy remains an outstanding challenge. Here we report a computational method for designing protein nanomaterials in which multiple copies of two distinct subunits co-assemble into a specific architecture. We use the method to design five 24-subunit cage-like protein nanomaterials in two distinct symmetric architectures and experimentally demonstrate that their structures are in close agreement with the computational design models. The accuracy of the method and the number and variety of two-component materials that it makes accessible suggest a route to the construction of functional protein nanomaterials tailored to specific applications.

Accurate design of co-assembling multi-component protein nanomaterials.,King NP, Bale JB, Sheffler W, McNamara DE, Gonen S, Gonen T, Yeates TO, Baker D Nature. 2014 Jun 5;510(7503):103-8. doi: 10.1038/nature13404. Epub 2014 May 25. PMID:24870237<ref>PMID:24870237</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4nwn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Campylobacter jejuni subsp. jejuni 81-176]]
[[Category: Large Structures]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium str. STm5]]
[[Category: Baker D]]
[[Category: Bale JB]]
[[Category: King NP]]
[[Category: McNamara DE]]
[[Category: Sheffler W]]
[[Category: Yeates TO]]