View source for 4nwz


==Structure of bacterial type II NADH dehydrogenase from Caldalkalibacillus thermarum at 2.5A resolution==
<StructureSection load='4nwz' size='340' side='right'caption='[[4nwz]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4nwz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Caldalkalibacillus_thermarum_TA2.A1 Caldalkalibacillus thermarum TA2.A1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NWZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NWZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4nwz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4nwz OCA], [https://pdbe.org/4nwz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4nwz RCSB], [https://www.ebi.ac.uk/pdbsum/4nwz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4nwz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F5L3B8_CALTT F5L3B8_CALTT] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Non-proton pumping type II NADH dehydrogenase (NDH-2) plays a central role in the respiratory metabolism of bacteria, and in the mitochondria of fungi, plants and protists. The lack of NDH-2 in mammalian mitochondria and its essentiality in important bacterial pathogens suggests these enzymes may represent a potential new drug target to combat microbial pathogens. Here, we report the first crystal structure of a bacterial NDH-2 enzyme at 2.5 A resolution from Caldalkalibacillus thermarum. The NDH-2 structure reveals a homodimeric organization that has a unique dimer interface. NDH-2 is localized to the cytoplasmic membrane by two separated C-terminal membrane-anchoring regions that are essential for membrane localization and FAD binding, but not NDH-2 dimerization. Comparison of bacterial NDH-2 with the yeast NADH dehydrogenase (Ndi1) structure revealed non-overlapping binding sites for quinone and NADH in the bacterial enzyme. The bacterial NDH-2 structure establishes a framework for the structure-based design of small-molecule inhibitors.

Structure of the bacterial type II NADH dehydrogenase: a monotopic membrane protein with an essential role in energy generation.,Heikal A, Nakatani Y, Dunn E, Weimar MR, Day CL, Baker EN, Lott JS, Sazanov LA, Cook GM Mol Microbiol. 2014 Jan 3. doi: 10.1111/mmi.12507. PMID:24444429<ref>PMID:24444429</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4nwz" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Caldalkalibacillus thermarum TA2 A1]]
[[Category: Large Structures]]
[[Category: Baker EN]]
[[Category: Cook GM]]
[[Category: Heikal A]]
[[Category: Lott JS]]
[[Category: Nakatani Y]]
[[Category: Sazanov LA]]