View source for 4okh


==Crystal structure of calpain-3 penta-EF-hand domain==
<StructureSection load='4okh' size='340' side='right'caption='[[4okh]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4okh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OKH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OKH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEU:2,5,8,11,14,17,20,23,26,29,32,35,38,41,44,47,50,53,56,59,62,65,68,71,74,77,80-HEPTACOSAOXADOOCTACONTAN-82-OL'>PEU</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4okh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4okh OCA], [https://pdbe.org/4okh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4okh RCSB], [https://www.ebi.ac.uk/pdbsum/4okh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4okh ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/CAN3_HUMAN CAN3_HUMAN] Autosomal recessive limb girdle muscular dystrophy type 2A. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
[https://www.uniprot.org/uniprot/CAN3_HUMAN CAN3_HUMAN] Calcium-regulated non-lysosomal thiol-protease.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Calpains are Ca(2+) dependent intracellular cysteine proteases that cleave a wide range of protein substrates to help implement Ca(2+) signaling in the cell. The major isoforms of this enzyme family, calpain-1 and calpain-2, are heterodimers of a large and a small subunit, with the main dimer interface being formed through their C-terminal penta-EF hand (PEF) domains. Calpain-3, or p94, is a skeletal muscle-specific isoform that is genetically linked to limb-girdle muscular dystrophy. Biophysical and modeling studies with the PEF domain of calpain-3 support the suggestion that full-length calpain-3 exists as a homodimer. Here, we report the crystallization of calpain-3's PEF domain and its crystal structure in the presence of Ca(2+) , which provides evidence for the homodimer architecture of calpain-3 and supports the molecular model that places a protease core at either end of the elongated dimer. Unlike other calpain PEF domain structures, the calpain-3 PEF domain contains a Ca(2+) bound at the EF5-hand used for homodimer association. Three of the four Ca(2+) -binding EF-hands of the PEF domains are concentrated near the protease core, and have the potential to radically change the local charge within the dimer during Ca(2+) signaling. Examination of the homodimer interface shows that there would be steric clashes if the calpain-3 large subunit were to try to pair with a calpain small subunit. Database Structural data are available in the Protein Data Bank database under accession number 4OKH. STRUCTURED DIGITAL ABSTRACT: Calpain-3 and Calpain-3 bind by x-ray crystallography (View interaction).

Crystal structure of calpain-3 penta-EF-hand (PEF) domain - a homodimerized PEF family member with calcium bound at the fifth EF-hand.,Partha SK, Ravulapalli R, Allingham JS, Campbell RL, Davies PL FEBS J. 2014 Jul;281(14):3138-49. doi: 10.1111/febs.12849. Epub 2014 Jun 9. PMID:24846670<ref>PMID:24846670</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4okh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Calpain 3D structures|Calpain 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Allingham JS]]
[[Category: Campbell RL]]
[[Category: Davies PL]]
[[Category: Karunan Partha S]]
[[Category: Ravulapalli R]]