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==A structural characterization of the isoniazid Mycobacterium tuberculosis drug target, Rv2971, in its unliganded form==
<StructureSection load='4otk' size='340' side='right'caption='[[4otk]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4otk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OTK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OTK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4otk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4otk OCA], [https://pdbe.org/4otk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4otk RCSB], [https://www.ebi.ac.uk/pdbsum/4otk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4otk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Y2971_MYCTU Y2971_MYCTU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aldo-keto reductases (AKR) are a large superfamily of NADPH-dependent oxidoreductases and play a role in detoxification of toxic metabolites. Rv2971, an AKR in Mycobacterium tuberculosis, has recently been identified as a target of isoniazid, a key first-line drug against tuberculosis. Here, the cloning, expression, purification, crystallization and structural characterization of Rv2971 are described. To gain insight into its function, the crystal structure of Rv2971 was successfully determined to 1.60 A resolution in its unliganded form. The structure exhibits a TIM-barrel fold typical of AKRs, revealing structural characteristics essential for function and substrate specificities, allowing a structural comparison between Rv2971 and other mycobacterial AKRs.

A structural characterization of the isoniazid Mycobacterium tuberculosis drug target, Rv2971, in its unliganded form.,Shahine A, Prasetyoputri A, Rossjohn J, Beddoe T Acta Crystallogr F Struct Biol Commun. 2014 May;70(Pt 5):572-7. doi:, 10.1107/S2053230X14007158. Epub 2014 Apr 17. PMID:24817712<ref>PMID:24817712</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4otk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mycobacterium tuberculosis]]
[[Category: Beddoe T]]
[[Category: Shahine A]]