View source for 4ouj


==Crystal structure of HA33B-Lac==
<StructureSection load='4ouj' size='340' side='right'caption='[[4ouj]], [[Resolution|resolution]] 1.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ouj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridium_botulinum_B1_str._Okra Clostridium botulinum B1 str. Okra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OUJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OUJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=PRD_900008:alpha-lactose'>PRD_900008</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ouj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ouj OCA], [https://pdbe.org/4ouj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ouj RCSB], [https://www.ebi.ac.uk/pdbsum/4ouj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ouj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B1INP8_CLOBK B1INP8_CLOBK] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Botulinum neurotoxins (BoNTs) are produced as progenitor toxin complexes (PTCs) by Clostridium botulinum. The PTCs are composed of BoNT and non-toxic neurotoxin-associated proteins (NAPs), which serve to protect and deliver BoNT through the gastrointestinal tract in food borne botulism. HA33 is a key NAP component that specifically recognizes host carbohydrates and helps enrich PTC on the intestinal lumen preceding its transport across the epithelial barriers. Here, we report the crystal structure of HA33 of type B PTC (HA33/B) in complex with lactose at 1.46A resolution. The structural comparisons among HA33 of serotypes A-D reveal two different HA33-glycan interaction modes. The glycan-binding pockets on HA33/A and B are more suitable to recognize galactose-containing glycans in comparison to the equivalent sites on HA33/C and D. On the contrary, HA33/C and D could potentially recognize Neu5Ac as an independent receptor, whereas HA33/A and B do not. These findings indicate that the different oral toxicity and host susceptibility observed among different BoNT serotypes could be partly determined by the serotype-specific interaction between HA33 and host carbohydrate receptors. Furthermore, we have identified a key structural water molecule that mediates the HA33/B-lactose interactions. It provides the structural basis for development of new receptor-mimicking compounds, which have enhanced binding affinity with HA33 through their water-displacing moiety.

High-resolution crystal structure of HA33 of botulinum neurotoxin type B progenitor toxin complex.,Lee K, Lam KH, Kruel AM, Perry K, Rummel A, Jin R Biochem Biophys Res Commun. 2014 Apr 4;446(2):568-73. doi:, 10.1016/j.bbrc.2014.03.008. Epub 2014 Mar 12. PMID:24631690<ref>PMID:24631690</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ouj" style="background-color:#fffaf0;"></div>

==See Also==
*[[Hemagglutinin 3D structures|Hemagglutinin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Clostridium botulinum B1 str. Okra]]
[[Category: Large Structures]]
[[Category: Jin R]]
[[Category: Lam K-H]]
[[Category: Lee K]]
[[Category: Perry K]]
[[Category: Rummel A]]