View source for 4pfu


==Crystal structure of mannobiose bound oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.05 A resolution==
<StructureSection load='4pfu' size='340' side='right'caption='[[4pfu]], [[Resolution|resolution]] 2.05&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pfu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PFU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PFU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.049&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRD_900115:4beta-beta-mannobiose'>PRD_900115</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pfu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pfu OCA], [https://pdbe.org/4pfu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pfu RCSB], [https://www.ebi.ac.uk/pdbsum/4pfu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pfu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G4FEC0_THEMA G4FEC0_THEMA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Many bacteria exist in a state of feast or famine where high nutrient availability leads to periods of growth followed by nutrient scarcity and growth stagnation. To adapt to the constantly changing nutrient flux, metabolite acquisition systems must be able to function over a broad range. This however creates difficulties as nutrient concentrations vary over many orders of magnitude, requiring metabolite acquisition systems to simultaneously balance ligand specificity and the dynamic range in which a response to a metabolite is elicited. Here we present how a gene duplication of a periplasmic binding protein (PBP) in a mannose ABC transport system potentially resolves this dilemma through gene functionalization. Determination of ligand binding affinities and specificities of the gene duplicates with fluorescence and circular dichroism demonstrate that although the binding specificity is maintained the Kd values for the same ligand differ over three orders of magnitude. These results suggest this metabolite acquisition system can transport ligand at both low and high environmental concentrations while preventing saturation with related and less preferentially metabolized compounds. The X-ray crystal structures of the beta mannose bound proteins help clarify the structural basis of gene functionalization and reveal that affinity and specificity are potentially encoded in different regions of the binding site. These studies suggest a possible functional role and adaptive advantage for the presence of two PBPs in ABC transport systems and a way bacteria can adapt to varying nutrient flux through functionalization of gene duplicates.

Duplication of genes in an ATP binding cassette transport system increases dynamic range while maintaining ligand specificity.,Ghimire-Rijal S, Lu X, Myles DA, Cuneo MJ J Biol Chem. 2014 Sep 10. pii: jbc.M114.590992. PMID:25210043<ref>PMID:25210043</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pfu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Cuneo MJ]]
[[Category: Ghimire-Rijal S]]
[[Category: Lu X]]