View source for 4pi9


==Crystal structure of S. Aureus Autolysin E in complex with muropeptide NAM-L-ALA-D-iGLU==
<StructureSection load='4pi9' size='340' side='right'caption='[[4pi9]], [[Resolution|resolution]] 1.48&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pi9]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_Mu50 Staphylococcus aureus subsp. aureus Mu50]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PI9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PI9 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.48&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3LT:(4R)-4-[[(2S)-2-[[(2R)-2-[(2R,3S,4R,5R,6R)-5-ACETAMIDO-2-(HYDROXYMETHYL)-3,6-BIS(OXIDANYL)OXAN-4-YL]OXYPROPANOYL]AMINO]PROPANOYL]AMINO]-5-AZANYL-5-OXIDANYLIDENE-PENTANOIC+ACID'>3LT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pi9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pi9 OCA], [https://pdbe.org/4pi9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pi9 RCSB], [https://www.ebi.ac.uk/pdbsum/4pi9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pi9 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0H3JT72_STAAM A0A0H3JT72_STAAM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Peptidoglycan is a giant molecule that forms the cell wall that surrounds bacterial cells. It is composed of alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) residues connected by beta-(1,4)-glycosidic bonds and cross-linked with short polypeptide chains. Owing to the increasing antibiotic resistance against drugs targeting peptidoglycan synthesis, studies of enzymes involved in the degradation of peptidoglycan, such as N-acetylglucos-aminidases, may expose new, valuable drug targets. The scientific challenge addressed here is how lysozymes, muramidases which are likely to be the most studied enzymes ever, and bacterial N-acetylglucosaminidases discriminate between two glycosidic bonds that are different in sequence yet chemically equivalent in the same NAG-NAM polymers. In spite of more than fifty years of structural studies of lysozyme, it is still not known how the enzyme selects the bond to be cleaved. Using macromolecular crystallography, chemical synthesis and molecular modelling, this study explains how these two groups of enzymes based on an equivalent structural core exhibit a difference in selectivity. The crystal structures of Staphylococcus aureusN-acetylglucosaminidase autolysin E (AtlE) alone and in complex with fragments of peptidoglycan revealed that N-acetylglucosaminidases and muramidases approach the substrate at alternate glycosidic bond positions from opposite sides. The recognition pocket for NAM residues in the active site of N-acetylglucosaminidases may make them a suitable drug target.

The mechanism behind the selection of two different cleavage sites in NAG-NAM polymers.,Mihelic M, Vlahovicek-Kahlina K, Renko M, Mesnage S, Dobersek A, Taler-Vercic A, Jakas A, Turk D IUCrJ. 2017 Feb 23;4(Pt 2):185-198. doi: 10.1107/S2052252517000367. eCollection, 2017 Mar 1. PMID:28250957<ref>PMID:28250957</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pi9" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus subsp. aureus Mu50]]
[[Category: Mihelic M]]
[[Category: Renko M]]
[[Category: Turk D]]