View source for 4pli


==Structure of the chromodomain of MRG2 in complex with H3K36me3==
<StructureSection load='4pli' size='340' side='right'caption='[[4pli]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pli]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PLI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PLI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.651&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=M3L:N-TRIMETHYLLYSINE'>M3L</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pli FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pli OCA], [https://pdbe.org/4pli PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pli RCSB], [https://www.ebi.ac.uk/pdbsum/4pli PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pli ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MRG2_ARATH MRG2_ARATH] Chromatin remodeling factor. Acts as a 'reader' protein by binding to H3K4me3 and H3K36me3 to control histone H4 acetylation. Increases the transcriptional levels of the flowering time genes FLC and FT (PubMed:25183522, PubMed:25211338). Binds the chromatin at the FT promoter upon interaction with CO (PubMed:25211338).<ref>PMID:25183522</ref> <ref>PMID:25211338</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Day-length is important for regulating the transition to reproductive development (flowering) in plants. In the model plant Arabidopsis thaliana, the transcription factor CONSTANS (CO) promotes expression of the florigen FLOWERING LOCUS T (FT), constituting a key flowering pathway under long-day photoperiods. Recent studies have revealed that FT expression is regulated by changes of histone modification marks of the FT chromatin, but the epigenetic regulators that directly interact with the CO protein have not been identified. Here, we show that the Arabidopsis Morf Related Gene (MRG) group proteins MRG1 and MRG2 act as H3K4me3/H3K36me3 readers and physically interact with CO to activate FT expression. In vitro binding analyses indicated that the chromodomains of MRG1 and MRG2 preferentially bind H3K4me3/H3K36me3 peptides. The mrg1 mrg2 double mutant exhibits reduced mRNA levels of FT, but not of CO, and shows a late-flowering phenotype under the long-day but not short-day photoperiod growth conditions. MRG2 associates with the chromatin of FT promoter in a way dependent of both CO and H3K4me3/H3K36me3. Vice versa, loss of MRG1 and MRG2 also impairs CO binding at the FT promoter. Crystal structure analyses of MRG2 bound with H3K4me3/H3K36me3 peptides together with mutagenesis analysis in planta further demonstrated that MRG2 function relies on its H3K4me3/H3K36me3-binding activity. Collectively, our results unravel a novel chromatin regulatory mechanism, linking functions of MRG1 and MRG2 proteins, H3K4/H3K36 methylations, and CO in FT activation in the photoperiodic regulation of flowering time in plants.

Regulation of arabidopsis flowering by the histone mark readers MRG1/2 via interaction with CONSTANS to modulate FT expression.,Bu Z, Yu Y, Li Z, Liu Y, Jiang W, Huang Y, Dong AW PLoS Genet. 2014 Sep 11;10(9):e1004617. doi: 10.1371/journal.pgen.1004617., eCollection 2014 Sep. PMID:25211338<ref>PMID:25211338</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pli" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Huang Y]]
[[Category: Liu Y]]