View source for 4pph


==Crystal structure of conglutin gamma, a unique basic 7S globulin from lupine seeds==
<StructureSection load='4pph' size='340' side='right'caption='[[4pph]], [[Resolution|resolution]] 2.01&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pph]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lupinus_angustifolius Lupinus angustifolius]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PPH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PPH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.009&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pph FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pph OCA], [https://pdbe.org/4pph PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pph RCSB], [https://www.ebi.ac.uk/pdbsum/4pph PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pph ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CONG1_LUPAN CONG1_LUPAN] Sulfur-rich seed storage protein that remains undegraded at germination.[UniProtKB:Q9FSH9]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
gamma-Conglutin from lupin seeds is an unusual 7S basic globulin protein. It is capable of reducing glycaemia in mammals, but the structural basis of this activity is not known. gamma-Conglutin shares a high level of structural homology with glycoside hydrolase inhibitor proteins, although it lacks any kind of inhibitory activity against plant cell-wall degradation enzymes. In addition, gamma-conglutin displays a less pronounced structural similarity to pepsin-like aspartic proteases, but it is proteolytically dysfunctional. Only one structural study of a legume 7S basic globulin, that isolated from soybean, has been reported to date. The quaternary assembly of soybean 7S basic globulin (Bg7S) is arranged as a cruciform-shaped tetramer comprised of two superposed dimers. Here, the crystal structure of gamma-conglutin isolated from Lupinus angustifolius seeds (LangC) is presented. The polypeptide chain of LangC is post-translationally cleaved into alpha and beta subunits but retains its covalent integrity owing to a disulfide bridge. The protomers of LangC undergo an intricate quaternary assembly, resulting in a ring-like hexamer with noncrystallographic D3 symmetry. The twofold-related dimers are similar to those in Bg7S but their assembly is different as a consequence of mutations in a beta-strand that is involved in intermolecular beta-sheet formation in gamma-conglutin. Structural elucidation of gamma-conglutin will help to explain its physiological role, especially in the evolutionary context, and will guide further research into the hypoglycaemic activity of this protein in humans, with potential consequences for novel antidiabetic therapies.

Structure of gamma-conglutin: insight into the quaternary structure of 7S basic globulins from legumes.,Czubinski J, Barciszewski J, Gilski M, Szpotkowski K, Debski J, Lampart-Szczapa E, Jaskolski M Acta Crystallogr D Biol Crystallogr. 2015 Feb;71(Pt 2):224-38. doi:, 10.1107/S1399004714025073. Epub 2015 Jan 23. PMID:25664733<ref>PMID:25664733</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pph" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lupinus angustifolius]]
[[Category: Barciszewski J]]
[[Category: Czubinski J]]
[[Category: Gilski M]]
[[Category: Jaskolski M]]
[[Category: Lampart-Szczapa E]]