View source for 4pqg


==Crystal structure of the pneumococcal O-GlcNAc transferase GtfA in complex with UDP and GlcNAc==
<StructureSection load='4pqg' size='340' side='right'caption='[[4pqg]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pqg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_TIGR4 Streptococcus pneumoniae TIGR4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PQG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pqg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqg OCA], [https://pdbe.org/4pqg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pqg RCSB], [https://www.ebi.ac.uk/pdbsum/4pqg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GTFA_STRPN GTFA_STRPN] Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.[UniProtKB:A0A0S4NM89]<ref>PMID:24936067</ref> <ref>PMID:28246170</ref> <ref>PMID:16861665</ref> 

==See Also==
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
*[[O-GlcNAc transferase 3D structures|O-GlcNAc transferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae TIGR4]]
[[Category: Chen Y]]
[[Category: Jiang YL]]
[[Category: Ren YM]]
[[Category: Shi WW]]
[[Category: Wu H]]
[[Category: Yang YH]]
[[Category: Zhou CZ]]
[[Category: Zhu F]]