View source for 4pqv


==Crystal structure of an Xrn1-resistant RNA from the 3' untranslated region of a flavivirus (Murray Valley Encephalitis virus)==
<StructureSection load='4pqv' size='340' side='right' caption='[[4pqv]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4pqv]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PQV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PQV FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pqv OCA], [http://pdbe.org/4pqv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pqv RCSB], [http://www.ebi.ac.uk/pdbsum/4pqv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pqv ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Flaviviruses are emerging human pathogens and worldwide health threats. During infection, pathogenic subgenomic flaviviral RNAs (sfRNAs) are produced by resisting degradation by the 5'--&gt;3' host cell exonuclease Xrn1 through an unknown RNA structure-based mechanism. Here, we present the crystal structure of a complete Xrn1-resistant flaviviral RNA, which contains interwoven pseudoknots within a compact structure that depends on highly conserved nucleotides. The RNA's three-dimensional topology creates a ringlike conformation, with the 5' end of the resistant structure passing through the ring from one side of the fold to the other. Disruption of this structure prevents formation of sfRNA during flaviviral infection. Thus, sfRNA formation results from an RNA fold that interacts directly with Xrn1, presenting the enzyme with a structure that confounds its helicase activity.

The structural basis of pathogenic subgenomic flavivirus RNA (sfRNA) production.,Chapman EG, Costantino DA, Rabe JL, Moon SL, Wilusz J, Nix JC, Kieft JS Science. 2014 Apr 18;344(6181):307-10. doi: 10.1126/science.1250897. PMID:24744377<ref>PMID:24744377</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4pqv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chapman, E G]]
[[Category: Costantino, D A]]
[[Category: Kieft, J S]]
[[Category: Moon, S L]]
[[Category: Nix, J C]]
[[Category: Rabe, J L]]
[[Category: Wilusz, J]]
[[Category: 3' untranslated region]]
[[Category: Exonuclease xrn1]]
[[Category: Flavivirus]]
[[Category: Pseudoknot]]
[[Category: Resists degradation]]
[[Category: Rna]]
[[Category: Sfrna]]
[[Category: Small flaviviral rna]]
[[Category: Type c rna three-way junction]]
[[Category: Xrn1 resistant rna]]