View source for 4ptw


==Halothermothrix orenii beta-glucosidase A, 2-deoxy-2-fluoro-glucose complex==
<StructureSection load='4ptw' size='340' side='right'caption='[[4ptw]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ptw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halothermothrix_orenii_H_168 Halothermothrix orenii H 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PTW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PTW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=G2F:2-DEOXY-2-FLUORO-ALPHA-D-GLUCOPYRANOSE'>G2F</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ptw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ptw OCA], [https://pdbe.org/4ptw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ptw RCSB], [https://www.ebi.ac.uk/pdbsum/4ptw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ptw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B8CYA8_HALOH B8CYA8_HALOH] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lactose is a major disaccharide by-product from the dairy industries, and production of whey alone amounts to about 200 million tons globally each year. Thus, it is of particular interest to identify improved enzymatic processes for lactose utilization. Microbial beta-glucosidases (BGL) with significant beta-galactosidase (BGAL) activity can be used to convert lactose to glucose (Glc) and galactose (Gal), and most retaining BGLs also synthesize more complex sugars from the monosaccharides by transglycosylation, such as galacto-oligosaccharides (GOS), which are prebiotic compounds that stimulate growth of beneficial gut bacteria. In this work, a BGL from the thermophilic and halophilic bacterium Halothermothrix orenii, HoBGLA, was characterized biochemically and structurally. It is an unspecific beta-glucosidase with mixed activities for different substrates and prominent activity with various galactosidases such as lactose. We show that HoBGLA is an attractive candidate for industrial lactose conversion based on its high activity and stability within a broad pH range (4.5-7.5), with maximal beta-galactosidase activity at pH 6.0. The temperature optimum is in the range of 65-70 degrees C, and HoBGLA also shows excellent thermostability at this temperature range. The main GOS products from HoBGLA transgalactosylation are beta-D-Galp-(1--&gt;6)-D-Lac (6GALA) and beta-D-Galp-(1--&gt;3)-D-Lac (3GALA), indicating that D-lactose is a better galactosyl acceptor than either of the monosaccharides. To evaluate ligand binding and guide GOS modeling, crystal structures of HoBGLA were determined in complex with thiocellobiose, 2-deoxy-2-fluoro-D-glucose and glucose. The two major GOS products, 3GALA and 6GALA, were modeled in the substrate-binding cleft of wild-type HoBGLA and shown to be favorably accommodated.

Biochemical and structural characterization of a thermostable beta-glucosidase from Halothermothrix orenii for galacto-oligosaccharide synthesis.,Hassan N, Nguyen TH, Intanon M, Kori LD, Patel BK, Haltrich D, Divne C, Tan TC Appl Microbiol Biotechnol. 2014 Aug 31. PMID:25173693<ref>PMID:25173693</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ptw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halothermothrix orenii H 168]]
[[Category: Large Structures]]
[[Category: Divne C]]
[[Category: Haltrich D]]
[[Category: Hassan N]]
[[Category: Kori LD]]
[[Category: Nguyen TH]]
[[Category: Patel BKC]]
[[Category: Tan TC]]