View source for 4q75


==Crystal structure of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana==
<StructureSection load='4q75' size='340' side='right'caption='[[4q75]], [[Resolution|resolution]] 1.71&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4q75]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Q75 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Q75 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.709&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CSS:S-MERCAPTOCYSTEINE'>CSS</scene>, <scene name='pdbligand=LLP:(2S)-2-AMINO-6-[[3-HYDROXY-2-METHYL-5-(PHOSPHONOOXYMETHYL)PYRIDIN-4-YL]METHYLIDENEAMINO]HEXANOIC+ACID'>LLP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4q75 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4q75 OCA], [https://pdbe.org/4q75 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4q75 RCSB], [https://www.ebi.ac.uk/pdbsum/4q75 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4q75 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CNIF1_ARATH CNIF1_ARATH] Catalyzes the removal of elemental sulfur and selenium atoms from L-cysteine, L-cystine, L-selenocysteine, and L-selenocystine to produce L-alanine (PubMed:12033984, PubMed:16455656). Supplies the inorganic sulfur for iron-sulfur (Fe-S) clusters (PubMed:15480755, PubMed:17372218). Required for the maturation of all plastidic Fe-S proteins and, thus, essential for plant growth (PubMed:17372218).<ref>PMID:12033984</ref> <ref>PMID:15480755</ref> <ref>PMID:16455656</ref> <ref>PMID:17372218</ref> 
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== Publication Abstract from PubMed ==
The chloroplastic Arabidopsis thaliana Nfs2 (AtNfs2) is a group II pyridoxal 5'-phosphate-dependent cysteine desulfurase that is involved in the initial steps of iron-sulfur cluster biogenesis. The group II cysteine desulfurases require the presence of sulfurtransferases such as SufE proteins for optimal activity. Compared with group I cysteine desulfurases, proteins of this group contains a smaller extended lobe harbouring the catalytic cysteine and have a beta-hairpin constraining the active site. Here, two crystal structures of AtNfs2 are reported: a wild-type form with the catalytic cysteine in a persulfide-intermediate state and a C384S variant mimicking the resting state of the enzyme. In both structures the well conserved Lys241 covalently binds pyridoxal 5'-phosphate, forming an internal aldimine. Based on available homologous bacterial complexes, a model of a complex between AtNfs2 and the SufE domain of its biological partner AtSufE1 is proposed, revealing the nature of the binding sites.

X-ray structures of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana.,Roret T, Pegeot H, Couturier J, Mulliert G, Rouhier N, Didierjean C Acta Crystallogr F Struct Biol Commun. 2014 Sep 1;70(Pt 9):1180-5. doi:, 10.1107/S2053230X14017026. Epub 2014 Aug 29. PMID:25195888<ref>PMID:25195888</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4q75" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cysteine desulfurase 3D structures|Cysteine desulfurase 3D structures]]
*[[Selenocysteine lyase|Selenocysteine lyase]]
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Didierjean C]]
[[Category: Roret T]]