View source for 4qqf


==Crystal structure of mitochondrial import inner membrane translocase subunit TIM50==
<StructureSection load='4qqf' size='340' side='right'caption='[[4qqf]], [[Resolution|resolution]] 2.67&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4qqf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4QQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4QQF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.672&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4qqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4qqf OCA], [https://pdbe.org/4qqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4qqf RCSB], [https://www.ebi.ac.uk/pdbsum/4qqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4qqf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TIM50_YEAST TIM50_YEAST] Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. Required to direct preproteins in transit and direct them to the channel protein TIM23, and possibly facilitates transfer of the translocating proteins from the TOM complex to the TIM23 complex. Does not act as a structural subunit of the TIM23 complex but plays a dynamic role in protein import. Has no phosphatase activity.<ref>PMID:12437924</ref> <ref>PMID:12437925</ref> <ref>PMID:14532110</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Mitochondrial preproteins are transported through the translocase of the outer membrane (TOM) complex. Tim50 and Tim23 then transfer preproteins with N-terminal targeting presequences through the intermembrane space (IMS) across the inner membrane. The crystal structure of the IMS domain of Tim50 [Tim50(164-361)] has previously been determined to 1.83 A resolution. Here, the crystal structure of Tim50(164-361) at 2.67 A resolution that was crystallized using a different condition is reported. Compared with the previously determined Tim50(164-361) structure, significant conformational changes occur within the protruding beta-hairpin of Tim50 and the nearby helix A2. These findings indicate that the IMS domain of Tim50 exhibits significant structural plasticity within the putative presequence-binding groove, which may play important roles in the function of Tim50 as a receptor protein in the TIM complex that interacts with the presequence and multiple other proteins. More interestingly, the crystal packing indicates that helix A1 from the neighboring monomer docks into the putative presequence-binding groove of Tim50(164-361), which may mimic the scenario of Tim50 and the presequence complex. Tim50 may recognize and bind the presequence helix by utilizing the inner side of the protruding beta-hairpin through hydrophobic interactions. Therefore, the protruding beta-hairpin of Tim50 may play critical roles in receiving the presequence and recruiting Tim23 for subsequent protein translocations.

The structure of Tim50(164-361) suggests the mechanism by which Tim50 receives mitochondrial presequences.,Li J, Sha B Acta Crystallogr F Struct Biol Commun. 2015 Sep;71(Pt 9):1146-51. doi:, 10.1107/S2053230X15013102. Epub 2015 Aug 25. PMID:26323300<ref>PMID:26323300</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4qqf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Li JZ]]