View source for 4r0m


==Structure of McyG A-PCP complexed with phenylalanyl-adenylate==
<StructureSection load='4r0m' size='340' side='right'caption='[[4r0m]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4r0m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Microcystis_aeruginosa_PCC_7806 Microcystis aeruginosa PCC 7806]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R0M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R0M FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FA5:ADENOSINE-5-[PHENYLALANINYL-PHOSPHATE]'>FA5</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r0m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r0m OCA], [https://pdbe.org/4r0m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r0m RCSB], [https://www.ebi.ac.uk/pdbsum/4r0m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r0m ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A8YJW1_MICA7 A8YJW1_MICA7] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Microcystins, which are the most common cause of hepatotoxicity associated with cyanobacterial water blooms, are assembled in vivo on a large multienzyme complex via a mixed nonribosomal peptide synthetase/polyketide synthetase (NRPS/PKS). The biosynthesis of microcystin in Microcystis aeruginosa PCC 7806 starts with the enzyme McyG, which contains an adenylation-peptidyl carrier protein (A-PCP) didomain for loading the starter unit to assemble the side chain of an Adda residue. However, the catalytic mechanism remains unclear. Here, the 2.45 A resolution crystal structure of the McyG A-PCP didomain complexed with the catalytic intermediate L-phenylalanyl-adenylate (L-Phe-AMP) is reported. Each asymmetric unit contains two protein molecules, one of which consists of the A-PCP didomain and the other of which comprises only the A domain. Structural analyses suggest that Val227 is likely to be critical for the selection of hydrophobic substrates. Moreover, two distinct interfaces demonstrating variable crosstalk between the PCP domain and the A domain were observed. A catalytic cycle for the adenylation and peptide transfer of the A-PCP didomain is proposed.

Structure of the adenylation-peptidyl carrier protein didomain of the Microcystis aeruginosa microcystin synthetase McyG.,Tan XF, Dai YN, Zhou K, Jiang YL, Ren YM, Chen Y, Zhou CZ Acta Crystallogr D Biol Crystallogr. 2015 Apr;71(Pt 4):873-81. doi:, 10.1107/S1399004715001716. Epub 2015 Mar 27. PMID:25849398<ref>PMID:25849398</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4r0m" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Microcystis aeruginosa PCC 7806]]
[[Category: Chen YX]]
[[Category: Dai YN]]
[[Category: Jiang YL]]
[[Category: Ren YM]]
[[Category: Tan XF]]
[[Category: Zhou CZ]]
[[Category: Zhou K]]