View source for 4r70


==Crystal structure of bacteriophytochrome RpBphP3 from photosynthetic bacterium R. palustris==
<StructureSection load='4r70' size='340' side='right'caption='[[4r70]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4r70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris_CGA009 Rhodopseudomonas palustris CGA009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R70 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r70 OCA], [https://pdbe.org/4r70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r70 RCSB], [https://www.ebi.ac.uk/pdbsum/4r70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r70 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
RpBphP2 and RpBphP3, two tandem bacteriophytochromes from the photosynthetic bacterium Rhodopseudomonas palustris, share high sequence identity but exhibit distinct photoconversion behavior. Unlike the canonical RpBphP2, RpBphP3 photoconverts to an unusual near-red-absorbing (Pnr) state; both are required for synthesis of light-harvesting complexes under low-light conditions. Here we report the crystal structures of the photosensory core modules of RpBphP2 and RpBphP3. Despite different quaternary structures, RpBphP2 and RpBphP3 adopt nearly identical tertiary structures. The RpBphP3 structure reveals tongue-and-groove interactions at the interface between the GAF and PHY domains. A single mutation in the PRxSF motif at the GAF-PHY interface abolishes light-induced formation of the Pnr state in RpBphP3, possibly due to altered structural rigidity of the chromophore-binding pocket. Structural comparisons suggest that long-range signaling involves structural rearrangement of the helical spine at the dimer interface. These structures, together with mutational studies, provide insights into photoconversion and the long-range signaling mechanism in phytochromes.

Light Signaling Mechanism of Two Tandem Bacteriophytochromes.,Yang X, Stojkovic EA, Ozarowski WB, Kuk J, Davydova E, Moffat K Structure. 2015 Jul 7;23(7):1179-89. doi: 10.1016/j.str.2015.04.022. Epub 2015, Jun 18. PMID:26095026<ref>PMID:26095026</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodopseudomonas palustris CGA009]]
[[Category: Kuk J]]
[[Category: Moffat K]]
[[Category: Yang X]]