View source for 4r83


==Crystal structure of Sialyltransferase from Photobacterium damsela==
<StructureSection load='4r83' size='340' side='right'caption='[[4r83]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4r83]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Photobacterium_damselae Photobacterium damselae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R83 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R83 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.93&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r83 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r83 OCA], [https://pdbe.org/4r83 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r83 RCSB], [https://www.ebi.ac.uk/pdbsum/4r83 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r83 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/O66375_9GAMM O66375_9GAMM] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Sialyltransferase structures fall into either GT-A or GT-B glycosyltransferase fold. Some sialyltransferases from the Photobacterium genus have been shown to contain an additional N-terminal immunoglobulin (Ig)-like domain. Photobacterium damselae alpha2-6-sialyltransferase has been used efficiently in enzymatic and chemoenzymatic synthesis of alpha2-6-linked sialosides. Here we report three crystal structures of this enzyme. Two structures with and without a donor substrate analog CMP-3F(a)Neu5Ac contain an immunoglobulin (Ig)-like domain and adopt the GT-B sialyltransferase fold. The binary structure reveals a non-productive pre-Michaelis complex, which are caused by crystal lattice contacts that prevent the large conformational changes. The third structure lacks the Ig-domain. Comparison of the three structures reveals small inherent flexibility between the two Rossmann-like domains of the GT-B fold.

Crystal structures of sialyltransferase from Photobacterium damselae.,Huynh N, Li Y, Yu H, Huang S, Lau K, Chen X, Fisher AJ FEBS Lett. 2014 Dec 20;588(24):4720-9. doi: 10.1016/j.febslet.2014.11.003. Epub, 2014 Nov 15. PMID:25451227<ref>PMID:25451227</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4r83" style="background-color:#fffaf0;"></div>

==See Also==
*[[Sialyltransferase 3D structures|Sialyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photobacterium damselae]]
[[Category: Chen X]]
[[Category: Fisher AJ]]
[[Category: Huynh N]]
[[Category: Li Y]]