View source for 4rh1


==Spore photoproduct lyase C140A/S76C mutant with bound AdoMet and dinucleoside spore photoproduct==
<StructureSection load='4rh1' size='340' side='right'caption='[[4rh1]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4rh1]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Geobacillus_thermodenitrificans_NG80-2 Geobacillus thermodenitrificans NG80-2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RH1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=0TT:1-[(2R,4S,5R)-5-(HYDROXYMETHYL)-4-OXIDANYL-OXOLAN-2-YL]-5-[[(5R)-1-[(2R,4S,5R)-5-(HYDROXYMETHYL)-4-OXIDANYL-OXOLAN-2-YL]-5-METHYL-2,4-BIS(OXIDANYLIDENE)-1,3-DIAZINAN-5-YL]METHYL]PYRIMIDINE-2,4-DIONE'>0TT</scene>, <scene name='pdbligand=EEM:[(3S)-3-AMINO-4-HYDROXY-4-OXO-BUTYL]-[[(2S,3S,4R,5R)-5-(6-AMINOPURIN-9-YL)-3,4-DIHYDROXY-OXOLAN-2-YL]METHYL]-METHYL-SELANIUM'>EEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rh1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh1 OCA], [https://pdbe.org/4rh1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rh1 RCSB], [https://www.ebi.ac.uk/pdbsum/4rh1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh1 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A4IQU1_GEOTN A4IQU1_GEOTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The radical SAM enzyme, spore photoproduct lyase, requires an H-atom transfer (HAT) pathway to catalyze DNA repair. By rational engineering, we demonstrate that it is possible to rewire its HAT pathway, a first step toward the development of novel catalysts based on the radical SAM enzyme scaffold.

Rescuing DNA repair activity by rewiring the H-atom transfer pathway in the radical SAM enzyme, spore photoproduct lyase.,Benjdia A, Heil K, Winkler A, Carell T, Schlichting I Chem Commun (Camb). 2014 Oct 6. PMID:25285338<ref>PMID:25285338</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rh1" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Geobacillus thermodenitrificans NG80-2]]
[[Category: Large Structures]]
[[Category: Benjdia A]]
[[Category: Carell T]]
[[Category: Heil K]]
[[Category: Schlichting I]]
[[Category: Winkler A]]