View source for 4ri7


==Crystal structure of poplar glutathione transferase F1 mutant SER 13 CYS==
<StructureSection load='4ri7' size='340' side='right'caption='[[4ri7]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ri7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Populus_tremula_x_Populus_tremuloides Populus tremula x Populus tremuloides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RI7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RI7 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.798&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GSH:GLUTATHIONE'>GSH</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ri7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ri7 OCA], [https://pdbe.org/4ri7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ri7 RCSB], [https://www.ebi.ac.uk/pdbsum/4ri7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ri7 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A9PHH6_POPTR A9PHH6_POPTR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glutathione transferases (GSTs) constitute a superfamily of enzymes with essential roles in cellular detoxification and secondary metabolism in plants as in other organisms. Several plant GSTs, including those of the Phi class (GSTFs), require a conserved catalytic serine residue to perform glutathione (GSH)-conjugation reactions. Genomic analyses revealed that terrestrial plants have around ten GSTFs, eight in the Populus trichocarpa genome, but their physiological functions and substrates are mostly unknown. Transcript expression analyses showed a predominant expression of all genes both in reproductive (female flowers, fruits, floral buds) and vegetative organs (leaves, petioles). Here, we show that the recombinant poplar GSTF1 (PttGSTF1) possesses peroxidase activity toward cumene hydroperoxide and GSH-conjugation activity toward model substrates such as 2,4-dinitrochlorobenzene, benzyl and phenetyl isothiocyanate, 4-nitrophenyl butyrate and 4-hydroxy-2-nonenal but interestingly not on previously identified GSTF-class substrates. In accordance with analytical gel filtration data, crystal structure of PttGSTF1 showed a canonical dimeric organization with bound GSH or 2-(N-morpholino)ethanesulfonic acid molecules. The structure of these protein-substrate complexes allowed delineating the residues contributing to both the G and H sites that form the active site cavity. In sum, the presence of GSTF1 transcripts and proteins in most poplar organs especially those rich in secondary metabolites such as flowers and fruits, together with its GSH-conjugation activity and its documented stress-responsive expression suggest that its function is associated with the catalytic transformation of metabolites and/or peroxide removal rather than with ligandin properties as previously reported for other GSTFs.

The poplar Phi class glutathione transferase: expression, activity and structure of GSTF1.,Pegeot H, Koh CS, Petre B, Mathiot S, Duplessis S, Hecker A, Didierjean C, Rouhier N Front Plant Sci. 2014 Dec 23;5:712. doi: 10.3389/fpls.2014.00712. eCollection, 2014. PMID:25566286<ref>PMID:25566286</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ri7" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Populus tremula x Populus tremuloides]]
[[Category: Didierjean C]]
[[Category: Mathiot S]]
[[Category: Pegeot H]]
[[Category: Rouhier N]]