View source for 4txv


==Crystal structure of the mixed disulfide intermediate between thioredoxin-like TlpAs(C110S) and subunit II of cytochrome c oxidase CoxBPD (C233S)==
<StructureSection load='4txv' size='340' side='right'caption='[[4txv]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4txv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bradyrhizobium_diazoefficiens_USDA_110 Bradyrhizobium diazoefficiens USDA 110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4TXV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4TXV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4txv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4txv OCA], [https://pdbe.org/4txv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4txv RCSB], [https://www.ebi.ac.uk/pdbsum/4txv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4txv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TLPA_BRADU TLPA_BRADU] Involved in cytochrome aa3 assembly.
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== Publication Abstract from PubMed ==
Two critical cysteine residues in the copper-A site (CuA) on subunit II (CoxB) of bacterial cytochrome c oxidase lie on the periplasmic side of the cytoplasmic membrane. As the periplasm is an oxidizing environment compared with the reducing cytoplasm, the prediction was that a disulfide bond formed between these cysteines must be eliminated by reduction prior to copper insertion. We show here that a periplasmic thioredoxin (TlpA) acts as a specific reductant not only for the Cu2+-transfer chaperone ScoI but also for CoxB. The dual role of TlpA was documented best with high-resolution crystal structures of the kinetically trapped TlpA-ScoI and TlpA-CoxB mixed-disulfide intermediates. They uncovered surprisingly disparate contact sites on TlpA for each of the two protein substrates. The equilibrium of CoxB reduction by TlpA revealed a thermodynamically favorable reaction, with a less negative redox potential of CoxB (E0' = -231 mV) compared with that of TlpA (E0' = -256 mV). The reduction of CoxB by TlpA via disulfide exchange proved to be very fast, with a rate constant of 8.4 x 104 M-1s-1 that is similar to that found previously for ScoI reduction. Hence, TlpA is a physiologically relevant reductase for both, ScoI and CoxB. While the requirement of ScoI for assembly of the CuA-CoxB complex may be bypassed in vivo by high environmental Cu2+ concentrations, TlpA is essential in this process because only reduced CoxB can bind copper ions.

How Periplasmic Thioredoxin TlpA Reduces Bacterial Copper Chaperone ScoI and Cytochrome Oxidase Subunit II (CoxB) Prior to Metallation.,Abicht HK, Schaerer MA, Quade N, Ledermann R, Mohorko E, Capitani G, Hennecke H, Glockshuber R J Biol Chem. 2014 Oct 1. pii: jbc.M114.607127. PMID:25274631<ref>PMID:25274631</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4txv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cytochrome c oxidase 3D structures|Cytochrome c oxidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bradyrhizobium diazoefficiens USDA 110]]
[[Category: Large Structures]]
[[Category: Abicht HK]]
[[Category: Glockshuber R]]
[[Category: Hennecke H]]
[[Category: Quade N]]