View source for 4u2a


==Structure of a lectin from the seeds of Vatairea macrocarpa complexed with GalNAc==
<StructureSection load='4u2a' size='340' side='right'caption='[[4u2a]], [[Resolution|resolution]] 1.74&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4u2a]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vatairea_macrocarpa Vatairea macrocarpa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2A OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2A FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=A2G:N-ACETYL-2-DEOXY-2-AMINO-GALACTOSE'>A2G</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2a OCA], [https://pdbe.org/4u2a PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2a RCSB], [https://www.ebi.ac.uk/pdbsum/4u2a PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2a ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/LECS_VATMA LECS_VATMA] Lectin that binds galactose.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plant lectins have been studied as histological markers and promising antineoplastic molecules for a long time, and structural characterization of different lectins bound to specific cancer epitopes has been carried out successfully. The crystal structures of Vatairea macrocarpa (VML) seed lectin in complex with GalNAc-alpha-O-Ser (Tn antigen) and GalNAc have been determined at the resolution of 1.4A and 1.7A, respectively. Molecular docking analysis of this new structure and other Tn-binding legume lectins to O-mucin fragments differently decorated with this antigen provides a comparative binding profile among these proteins, stressing that subtle alterations that may not influence monosaccharide binding can, nonetheless, directly impact the ability of these lectins to recognize naturally occurring antigens. In addition to the specific biological effects of VML, the structural and binding similarities between it and other lectins commonly used as histological markers (e.g., VVLB4 and SBA) strongly suggest VML as a candidate tool for cancer research.

High-resolution structure of a new Tn antigen-binding lectin from Vatairea macrocarpa and a comparative analysis of Tn-binding legume lectins.,de Sousa BL, da Silva Filho JC, Kumar P, Pereira RI, Lyskowski A, da Rocha BA, Delatorre P, Bezerra GA, Nagano CS, Gruber K, Cavada BS Int J Biochem Cell Biol. 2014 Dec 12. pii: S1357-2725(14)00385-9. doi:, 10.1016/j.biocel.2014.12.002. PMID:25499445<ref>PMID:25499445</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4u2a" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Vatairea macrocarpa]]
[[Category: Bezerra GA]]
[[Category: Cavada BS]]
[[Category: Delatorre P]]
[[Category: Gruber K]]
[[Category: Kumar P]]
[[Category: Lyskowski A]]
[[Category: Nagano CS]]
[[Category: Rocha BAM]]
[[Category: Silva-Filho JC]]
[[Category: Sousa BL]]