View source for 4u2w


==Atomic resolution crystal structure of HV-BBI protease inhibitor from amphibian skin in complex with bovine trypsin==
<StructureSection load='4u2w' size='340' side='right'caption='[[4u2w]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4u2w]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus] and [https://en.wikipedia.org/wiki/Odorrana_versabilis Odorrana versabilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U2W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U2W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u2w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u2w OCA], [https://pdbe.org/4u2w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u2w RCSB], [https://www.ebi.ac.uk/pdbsum/4u2w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u2w ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B1VC43_ODOVE B1VC43_ODOVE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Protease inhibitors of the Bowman-Birk (BBI) family are commonly found in plants and animals where they play a protective role against invading pathogens. Here we report an atomic resolution (1A) crystal structure of a peptide inhibitor isolated from a skin secretion of a Chinese bamboo odorous frog Huia versabilis (HV-BBI) in complex with trypsin. HV-BBI shares significant similarities in sequence with a previously described inhibitor from a diskless-fingered odorous frog Odorrana graham (ORB). However, the latter is characterized by more than a 16000 fold higher Ki against trypsin than HV-BBI. Comparative analysis of trypsin co-crystal structures of HV-BBI and ORB and additionally that of Sunflower Trypsin Inhibitor (SFTI-1) together with accessory information on the affinities of inhibitor variants allowed us to pinpoint the inhibitor moiety responsible for the observed large difference in activity and also to define the extent of modifications permissible within the common protease-binding loop scaffold of BBI inhibitors. We suggest that modifications outside of the inhibitory loop permit the evolution of specificity towards different enzymes characterized by trypsin-like specificity. This article is protected by copyright. All rights reserved.

Atomic resolution crystal structure of HV-BBI protease inhibitor from amphibian skin in complex with bovine trypsin.,Grudnik P, Debowski D, Legowska A, Malicki S, Golik P, Karna N, Rolka K, Dubin G Proteins. 2014 Dec 24. doi: 10.1002/prot.24750. PMID:25546528<ref>PMID:25546528</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4u2w" style="background-color:#fffaf0;"></div>

==See Also==
*[[Trypsin 3D structures|Trypsin 3D structures]]
*[[Trypsin inhibitor 3D structures|Trypsin inhibitor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bos taurus]]
[[Category: Large Structures]]
[[Category: Odorrana versabilis]]
[[Category: Debowski D]]
[[Category: Dubin G]]
[[Category: Golik P]]
[[Category: Grudnik P]]
[[Category: Legowska A]]
[[Category: Malicki S]]
[[Category: Rolka K]]