View source for 4u35


==Crystal Structures of RNA Duplexes Containing 2-thio-Uridine==
<StructureSection load='4u35' size='340' side='right'caption='[[4u35]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4u35]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4U35 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4U35 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SUR:1-(BETA-D-RIBOFURANOSYL)-2-THIO-URACIL-5-PHOSPHATE'>SUR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4u35 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4u35 OCA], [https://pdbe.org/4u35 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4u35 RCSB], [https://www.ebi.ac.uk/pdbsum/4u35 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4u35 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Structural studies of modified nucleobases in RNA duplexes are critical for developing a full understanding of the stability and specificity of RNA base pairing. 2-Thio-uridine (s2U) is a modified nucleobase found in certain tRNAs. Thermodynamic studies have evaluated the effects of s2U on base pairing in RNA, where it has been shown to stabilize U:A pairs and destabilize U:G wobble pairs. Surprisingly, no high-resolution crystal structures of s2U-containing RNA duplexes have yet been reported. We present here two high-resolution crystal structures of heptamer RNA duplexes (5'-uagcs2Ucc-3' paired with 3'-aucgAgg-5' and with 3'-aucgUgg-5') containing s2U:A and s2U:U pairs, respectively. For comparison, we also present the structures of their native counterparts solved under identical conditions. We found that replacing O2 with S2 stabilizes the U:A base pair without any detectable structural perturbation. In contrast, an s2U:U base pair is strongly stabilized in one specific U:U pairing conformation out of four observed for the native U:U base pair. This s2U:U stabilization appears to be due at least in part to an unexpected sulfur-mediated hydrogen bond. This work provides additional insights into the effects of 2-thio-uridine on RNA base pairing.

Crystal Structure Studies of RNA Duplexes Containing sU:A and sU:U Base Pairs.,Sheng J, Larsen A, Heuberger BD, Blain JC, Szostak JW J Am Chem Soc. 2014 Sep 16. PMID:25188906<ref>PMID:25188906</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4u35" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Blain JC]]
[[Category: Heuberger B]]
[[Category: Larsen A]]
[[Category: Sheng J]]
[[Category: Szostak JW]]