View source for 4ufq


==Structure of a novel Hyaluronidase (Hyal_Sk) from Streptomyces koganeiensis.==
<StructureSection load='4ufq' size='340' side='right'caption='[[4ufq]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ufq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_koganeiensis Streptomyces koganeiensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UFQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UFQ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ufq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ufq OCA], [https://pdbe.org/4ufq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ufq RCSB], [https://www.ebi.ac.uk/pdbsum/4ufq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ufq ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0U2E2J7_9ACTN A0A0U2E2J7_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Hyaluronidases (Hyals) are broadly used in medical applications to facilitate the dispersion and/or absorption of fluids or medications. This study reports the isolation, cloning, and industrial-scale recombinant production, purification and full characterization, including X-ray structure determination at 1.45 A, of an extracellular Hyal from the nonpathogenic bacterium Streptomyces koganeiensis. The recombinant S. koganeiensis Hyal (rHyal_Sk) has a novel bacterial catalytic domain with high enzymatic activity, compared with commercially available Hyals, and is more thermostable and presents higher proteolytic resistance, with activity over a broad pH range. Moreover, rHyal_Sk exhibits remarkable substrate specificity for hyaluronic acid (HA) and poses no risk of animal cross-infection.

Identification and characterization of a bacterial hyaluronidase and its production in recombinant form.,Messina L, Gavira JA, Pernagallo S, Unciti-Broceta JD, Sanchez Martin RM, Diaz-Mochon JJ, Vaccaro S, Conejero-Muriel M, Pineda-Molina E, Caruso S, Musumeci L, Di Pasquale R, Pontillo A, Sincinelli F, Pavan M, Secchieri C FEBS Lett. 2016 Jun 17. doi: 10.1002/1873-3468.12258. PMID:27311405<ref>PMID:27311405</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4ufq" style="background-color:#fffaf0;"></div>

==See Also==
*[[Hyaluronidase 3D structures|Hyaluronidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces koganeiensis]]
[[Category: Bisicchia S]]
[[Category: Caruso S]]
[[Category: Conejero-Muriel M]]
[[Category: Di Pasquale R]]
[[Category: Diaz-Mochon JJ]]
[[Category: Gavira JA]]
[[Category: Messina L]]
[[Category: Musumeci L]]
[[Category: Pernagallo S]]
[[Category: Unciti-Broceta JD]]
[[Category: Vaccaro S]]