View source for 4uoi


==Unexpected structure for the N-terminal domain of Hepatitis C virus envelope glycoprotein E1==
<StructureSection load='4uoi' size='340' side='right'caption='[[4uoi]], [[Resolution|resolution]] 3.49&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uoi]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Hepatitis_C_virus_(isolate_H77) Hepatitis C virus (isolate H77)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UOI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UOI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.49&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uoi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uoi OCA], [https://pdbe.org/4uoi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uoi RCSB], [https://www.ebi.ac.uk/pdbsum/4uoi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uoi ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H9XGD6_9HEPC H9XGD6_9HEPC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Single-wavelength anomalous dispersion of S atoms (S-SAD) is an elegant phasing method to determine crystal structures that does not require heavy-atom incorporation or selenomethionine derivatization. Nevertheless, this technique has been limited by the paucity of the signal at the usual X-ray wavelengths, requiring very accurate measurement of the anomalous differences. Here, the data collection and structure solution of the N-terminal domain of the ectodomain of HCV E1 from crystals that diffracted very weakly is reported. By combining the data from 32 crystals, it was possible to solve the sulfur substructure and calculate initial maps at 7 A resolution, and after density modication and phase extension using a higher resolution native data set to 3.5 A resolution model building was achievable.

Pushing the limits of sulfur SAD phasing: de novo structure solution of the N-terminal domain of the ectodomain of HCV E1.,El Omari K, Iourin O, Kadlec J, Fearn R, Hall DR, Harlos K, Grimes JM, Stuart DI Acta Crystallogr D Biol Crystallogr. 2014 Aug 1;70(Pt 8):2197-203. doi:, 10.1107/S139900471401339X. Epub 2014 Jul 25. PMID:25084338<ref>PMID:25084338</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uoi" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: El Omari K]]
[[Category: Grimes JM]]
[[Category: Harlos K]]
[[Category: Iourin O]]
[[Category: Kadlec J]]
[[Category: Stuart DI]]