View source for 4uow


==Crystal structure of the titin M10-Obscurin Ig domain 1 complex==
<StructureSection load='4uow' size='340' side='right'caption='[[4uow]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uow]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UOW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UOW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uow FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uow OCA], [https://pdbe.org/4uow PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uow RCSB], [https://www.ebi.ac.uk/pdbsum/4uow PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uow ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OBSCN_HUMAN OBSCN_HUMAN] Involved in myofibrillogenesis. Seems to be involved in assembly of myosin into sarcomeric A bands in striated muscle. Isoform 3 together with ANK1 isoform Mu17/Ank1.5 may provide a molecular link between the sarcoplasmic reticulum and myofibrils.<ref>PMID:11448995</ref> <ref>PMID:16205939</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
M10 is the most C-terminal immunoglobulin (Ig) domain of the giant protein titin and a frequent target of disease-linked mutations. Currently, it is the only known muscle Ig-domain able to interact with two alternative ligands - obscurin and obscurin-like-1 (Obsl1) - in different sarcomeric subregions. Obscurin and Obsl1 use their homologous N-terminal Ig domain (O1 in obscurin and OL1 in Obsl1) to bind M10 in a mutually exclusive manner. We present here the X-ray structure of the human titin:obscurin M10:O1 complex extending our previous work on the M10:OL1 interaction. Similar to M10:OL1, the M10:O1 complex displays a chevron-shaped antiparallel Ig-Ig architecture held together by a conserved molecular interface, which we validated by isothermal titration calorimetry and sorting experiments in neonatal rat cardiomyocytes (NRCs). O1 although structurally related to OL1 and M10, both members of the I-set Ig family, presents an intriguing switch of its betaA' strand. This leads to structural differences between the complexes, particularly, for the 'open-side' of the chevron-shaped assembly. A bioinformatics analysis reveals that the betaA'-switch observed for O1 is rare and that it is involved in mediating protein-protein interactions. Molecular Dynamics simulations also suggest that this topological alteration substantially increases local flexibility compared to the conventional I-set Ig domains. The O1/OL1 Ig domains are candidate discriminatory structural modules potentially directing the binding of specific additional partners at the M-band. Cellular sorting experiments in NRCs are consistent with the view that the titin:obscurin/Obsl1 complexes might be a platform for higher order interactions.

The Crystal Structure of the Human Titin:Obscurin Complex Reveals a Conserved Yet Specific Muscle M-band Zipper Module.,Pernigo S, Fukuzawa A, Pandini A, Holt M, Kleinjung J, Gautel M, Steiner RA J Mol Biol. 2014 Dec 6. pii: S0022-2836(14)00615-9. doi:, 10.1016/j.jmb.2014.11.019. PMID:25490259<ref>PMID:25490259</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uow" style="background-color:#fffaf0;"></div>

==See Also==
*[[Obscurin|Obscurin]]
*[[Titin 3D structures|Titin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Fukuzawa A]]
[[Category: Gautel M]]
[[Category: Pernigo S]]
[[Category: Steiner RA]]