View source for 4uqf


==CRYSTAL STRUCTURE OF LISTERIA MONOCYTOGENES GTP CYCLOHYDROLASE I==
<StructureSection load='4uqf' size='340' side='right'caption='[[4uqf]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uqf]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Listeria_monocytogenes Listeria monocytogenes]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UQF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UQF FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uqf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uqf OCA], [https://pdbe.org/4uqf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uqf RCSB], [https://www.ebi.ac.uk/pdbsum/4uqf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uqf ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCH1_LISMO GCH1_LISMO] 
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== Publication Abstract from PubMed ==
A putative open reading frame encoding GTP cyclohydrolase I from Listeria monocytogenes was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified and was confirmed to convert GTP to dihydroneopterin triphosphate (Km = 53 microM; vmax = 180 nmol mg(-1) min(-1)). The protein was crystallized from 1.3 M sodium citrate pH 7.3 and the crystal structure was solved at a resolution of 2.4 A (Rfree = 0.226) by molecular replacement using human GTP cyclohydrolase I as a template. The protein is a D5-symmetric decamer with ten topologically equivalent active sites. Screening a small library of about 9000 compounds afforded several inhibitors with IC50 values in the low-micromolar range. Several inhibitors had significant selectivity with regard to human GTP cyclohydrolase I. Hence, GTP cyclohydrolase I may be a potential target for novel drugs directed at microbial infections, including listeriosis, a rare disease with high mortality.

Structure of GTP cyclohydrolase I from Listeria monocytogenes, a potential anti-infective drug target.,Schussler S, Haase I, Perbandt M, Illarionov B, Siemens A, Richter K, Bacher A, Fischer M, Grawert T Acta Crystallogr F Struct Biol Commun. 2019 Sep 1;75(Pt 9):586-592. doi:, 10.1107/S2053230X19010902. Epub 2019 Aug 30. PMID:31475925<ref>PMID:31475925</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4uqf" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cyclohydrolase 3D structures|Cyclohydrolase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Listeria monocytogenes]]
[[Category: Fischer M]]
[[Category: Graewert T]]
[[Category: Perbandt M]]
[[Category: Schuessler S]]