View source for 4uri


==Crystal structure of chitinase-like agglutinin RobpsCRA from Robinia pseudoacacia==
<StructureSection load='4uri' size='340' side='right'caption='[[4uri]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uri]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Robinia_pseudoacacia Robinia pseudoacacia]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4URI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4URI FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uri FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uri OCA], [https://pdbe.org/4uri PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uri RCSB], [https://www.ebi.ac.uk/pdbsum/4uri PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uri ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A1YZD2_ROBPS A1YZD2_ROBPS] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
A new chitinase-like agglutinin, RobpsCRA, related to family GH18 chitinases, has previously been identified in black locust (Robinia pseudoacacia) bark. The crystal structure of RobpsCRA at 1.85A resolution reveals unusual molecular determinants responsible for the lack of its ancestral chitinase activity. Unlike other chitinase-like proteins, which lack chitinase catalytic residues, RobpsCRA has conserved its catalytic machinery. However, concerted rearrangements of loop regions coupled to non-conservative substitutions of aromatic residues central to the chitin-binding groove explain the lack of hydrolytic activity against chitin and the switch toward recognition of high-mannose type N-glycans. Identification of close homologs in flowering plants with conservation of sequence motifs associated to the structural adaptations seen in RobpsCRA defines an emerging class of agglutinins, as emphasized by a phylogenetic analysis, that are likely to share a similar carbohydrate binding specificity for high-mannose type N-glycans. This study illustrates the recent evolution and molecular adaptation of a versatile TIM-barrel scaffold within the ancestral GH18 family.

Structural basis for carbohydrate binding properties of a plant chitinase-like agglutinin with conserved catalytic machinery.,Sulzenbacher G, Roig-Zamboni V, Peumans WJ, Henrissat B, van Damme EJ, Bourne Y J Struct Biol. 2015 Feb 26. pii: S1047-8477(15)00029-5. doi:, 10.1016/j.jsb.2015.01.013. PMID:25727185<ref>PMID:25727185</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uri" style="background-color:#fffaf0;"></div>

==See Also==
*[[Agglutinin 3D structures|Agglutinin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Robinia pseudoacacia]]
[[Category: Bourne Y]]
[[Category: Henrissat B]]
[[Category: Peumans WJ]]
[[Category: Roig-Zamboni V]]
[[Category: Sulzenbacher G]]
[[Category: Van Damme EJM]]