View source for 4uzg


==Crystal structure of group B streptococcus pilus 2b backbone protein SAK_1440==
<StructureSection load='4uzg' size='340' side='right'caption='[[4uzg]], [[Resolution|resolution]] 1.06&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4uzg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_agalactiae_A909 Streptococcus agalactiae A909]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4UZG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4UZG FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.06&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4uzg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4uzg OCA], [https://pdbe.org/4uzg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4uzg RCSB], [https://www.ebi.ac.uk/pdbsum/4uzg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4uzg ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0M3KKY8_STRA1 A0A0M3KKY8_STRA1] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Group B Streptococcus (GBS) is a major cause of invasive disease in infants. Like other Gram-positive bacteria, GBS uses a sortase C-catalyzed transpeptidation mechanism to generate cell surface pili from backbone and ancillary pilin precursor substrates. The three pilus types identified in GBS contain structural subunits that are highly immunogenic and are promising candidates for the development of a broadly-protective vaccine. Here we report the X-ray crystal structure of the backbone protein of pilus 2b (BP-2b) at 1.06A resolution. The structure reveals a classical IgG-like fold typical of the pilin subunits of other Gram-positive bacteria. The crystallized portion of the protein (residues 185-468) encompasses domains D2 and D3 that together confer high stability to the protein due to the presence of an internal isopeptide bond within each domain. The D2+D3 region, lacking the N-terminal D1 domain, was as potent as the entire protein in conferring protection against GBS challenge in a well-established mouse model. By site-directed mutagenesis and complementation studies in GBS knock-out strains we identified the residues and motives essential for assembly of the BP-2b monomers into high-molecular weight complexes, thus providing new insights into pilus 2b polymerization.

Structure and assembly of group B streptococcus pilus 2b backbone protein.,Cozzi R, Malito E, Lazzarin M, Nuccitelli A, Castagnetti A, Bottomley MJ, Margarit I, Maione D, Rinaudo CD PLoS One. 2015 May 5;10(5):e0125875. doi: 10.1371/journal.pone.0125875., eCollection 2015. PMID:25942637<ref>PMID:25942637</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4uzg" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus agalactiae A909]]
[[Category: Bottomley MJ]]
[[Category: Cozzi R]]
[[Category: Malito E]]