View source for 4v00


==Structural and functional characterization of a novel monotreme- specific protein from the milk of the platypus==
<StructureSection load='4v00' size='340' side='right'caption='[[4v00]], [[Resolution|resolution]] 1.82&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v00]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ornithorhynchus_anatinus Ornithorhynchus anatinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V00 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V00 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v00 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v00 OCA], [https://pdbe.org/4v00 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v00 RCSB], [https://www.ebi.ac.uk/pdbsum/4v00 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v00 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/F6UME2_ORNAN F6UME2_ORNAN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Monotreme lactation protein (MLP) is a recently identified protein with antimicrobial activity. It is present in the milk of monotremes and is unique to this lineage. To characterize MLP and to gain insight into the potential role of this protein in the evolution of lactation, the crystal structure of duck-billed platypus (Ornithorhynchus anatinus) MLP was determined at 1.82 A resolution. This is the first structure to be reported for this novel, mammalian antibacterial protein. MLP was expressed as a FLAG epitope-tagged protein in mammalian cells and crystallized readily, with at least three space groups being observed (P1, C2 and P21). A 1.82 A resolution native data set was collected from a crystal in space group P1, with unit-cell parameters a = 51.2, b = 59.7, c = 63.1 A, alpha = 80.15, beta = 82.98, gamma = 89.27 degrees . The structure was solved by SAD phasing using a protein crystal derivatized with mercury in space group C2, with unit-cell parameters a = 92.7, b = 73.2, c = 56.5 A, beta = 90.28 degrees . MLP comprises a monomer of 12 helices and two short beta-strands, with much of the N-terminus composed of loop regions. The crystal structure of MLP reveals no three-dimensional similarity to any known structures and reveals a heretofore unseen fold, supporting the idea that monotremes may be a rich source for the identification of novel proteins. It is hypothesized that MLP in monotreme milk has evolved to specifically support the unusual lactation strategy of this lineage and may have played a central role in the evolution of these mammals.

Structural characterization of a novel monotreme-specific protein with antimicrobial activity from the milk of the platypus.,Newman J, Sharp JA, Enjapoori AK, Bentley J, Nicholas KR, Adams TE, Peat TS Acta Crystallogr F Struct Biol Commun. 2018 Jan 1;74(Pt 1):39-45. doi:, 10.1107/S2053230X17017708. Epub 2018 Jan 1. PMID:29372906<ref>PMID:29372906</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v00" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ornithorhynchus anatinus]]
[[Category: Adams TE]]
[[Category: Enjapoori AK]]
[[Category: Newman J]]
[[Category: Nicholas KR]]
[[Category: Peat TS]]
[[Category: Polekina G]]
[[Category: Sharp JA]]