View source for 4v7v


==Crystal structure of the E. coli ribosome bound to clindamycin.==
<StructureSection load='4v7v' size='340' side='right'caption='[[4v7v]], [[Resolution|resolution]] 3.29&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4v7v]] is a 18 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3ofx 3ofx], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3ofy 3ofy], [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3ofz 3ofz] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3og0 3og0]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V7V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4V7V FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CLY:CLINDAMYCIN'>CLY</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4v7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v7v OCA], [https://pdbe.org/4v7v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4v7v RCSB], [https://www.ebi.ac.uk/pdbsum/4v7v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4v7v ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RS2_ECOLI RS2_ECOLI] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Differences between the structures of bacterial, archaeal, and eukaryotic ribosomes account for the selective action of antibiotics. Even minor variations in the structure of ribosomes of different bacterial species may lead to idiosyncratic, species-specific interactions of the drugs with their targets. Although crystallographic structures of antibiotics bound to the peptidyl transferase center or the exit tunnel of archaeal (Haloarcula marismortui) and bacterial (Deinococcus radiodurans) large ribosomal subunits have been reported, it remains unclear whether the interactions of antibiotics with these ribosomes accurately reflect those with the ribosomes of pathogenic bacteria. Here we report X-ray crystal structures of the Escherichia coli ribosome in complexes with clinically important antibiotics of four major classes, including the macrolide erythromycin, the ketolide telithromycin, the lincosamide clindamycin, and a phenicol, chloramphenicol, at resolutions of approximately 3.3 A-3.4 A. Binding modes of three of these antibiotics show important variations compared to the previously determined structures. Biochemical and structural evidence also indicates that interactions of telithromycin with the E. coli ribosome more closely resembles drug binding to ribosomes of bacterial pathogens. The present data further argue that the identity of nucleotides 752, 2609, and 2055 of 23S ribosomal RNA explain in part the spectrum and selectivity of antibiotic action.

Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action.,Dunkle JA, Xiong L, Mankin AS, Cate JH Proc Natl Acad Sci U S A. 2010 Oct 5;107(40):17152-7. Epub 2010 Sep 27. PMID:20876128<ref>PMID:20876128</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4v7v" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli K-12]]
[[Category: Large Structures]]
[[Category: Cate JHD]]
[[Category: Dunkle JA]]
[[Category: Mankin AS]]
[[Category: Xiong L]]