View source for 4wla


==Time Resolved Serial Femtosecond Crystallography Captures High Resolution Intermediates of PYP==
<StructureSection load='4wla' size='340' side='right'caption='[[4wla]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wla]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halorhodospira_halophila Halorhodospira halophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WLA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WLA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HC4:4-HYDROXYCINNAMIC+ACID'>HC4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wla FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wla OCA], [https://pdbe.org/4wla PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wla RCSB], [https://www.ebi.ac.uk/pdbsum/4wla PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wla ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/PYP_HALHA PYP_HALHA] Photoactive blue light protein. Probably functions as a photoreceptor for a negative phototaxis response.
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== Publication Abstract from PubMed ==
Serial femtosecond crystallography using ultrashort pulses from x-ray free electron lasers (XFELs) enables studies of the light-triggered dynamics of biomolecules. We used microcrystals of photoactive yellow protein (a bacterial blue light photoreceptor) as a model system and obtained high-resolution, time-resolved difference electron density maps of excellent quality with strong features; these allowed the determination of structures of reaction intermediates to a resolution of 1.6 angstroms. Our results open the way to the study of reversible and nonreversible biological reactions on time scales as short as femtoseconds under conditions that maximize the extent of reaction initiation throughout the crystal.

Time-resolved serial crystallography captures high-resolution intermediates of photoactive yellow protein.,Tenboer J, Basu S, Zatsepin N, Pande K, Milathianaki D, Frank M, Hunter M, Boutet S, Williams GJ, Koglin JE, Oberthuer D, Heymann M, Kupitz C, Conrad C, Coe J, Roy-Chowdhury S, Weierstall U, James D, Wang D, Grant T, Barty A, Yefanov O, Scales J, Gati C, Seuring C, Srajer V, Henning R, Schwander P, Fromme R, Ourmazd A, Moffat K, Van Thor JJ, Spence JC, Fromme P, Chapman HN, Schmidt M Science. 2014 Dec 5;346(6214):1242-6. doi: 10.1126/science.1259357. PMID:25477465<ref>PMID:25477465</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wla" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Halorhodospira halophila]]
[[Category: Large Structures]]
[[Category: Schmidt M]]
[[Category: Tenboer J]]