View source for 4wlk


==Stationary Phase Survival Protein YuiC from B.subtilis complexed with reaction product==
<StructureSection load='4wlk' size='340' side='right'caption='[[4wlk]], [[Resolution|resolution]] 2.03&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wlk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WLK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WLK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3QL:N-[(1R,2S,3R,4R,5R)-2-[(2S,3R,4R,5S,6R)-3-ACETAMIDO-6-(HYDROXYMETHYL)-4,5-BIS(OXIDANYL)OXAN-2-YL]OXY-3-OXIDANYL-6,8-DIOXABICYCLO[3.2.1]OCTAN-4-YL]ETHANAMIDE'>3QL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wlk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wlk OCA], [https://pdbe.org/4wlk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wlk RCSB], [https://www.ebi.ac.uk/pdbsum/4wlk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wlk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YUIC_BACSU YUIC_BACSU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Stationary phase survival proteins (Sps) were found in Firmicutes as having analogous domain compositions, and in some cases genome context, as the resuscitation promoting factors of Actinobacteria, but with a different putative peptidoglycan cleaving domain. RESULTS: The first structure of a Firmicute Sps protein YuiC from B. subtilis, is found to be a stripped down version of the cell-wall peptidoglycan hydrolase MltA. The YuiC structures are of a domain swapped dimer, although some monomer is also found in solution. The protein crystallised in the presence of pentasaccharide shows a 1,6-anhydrodisaccharide sugar product, indicating that YuiC cleaves the sugar backbone to form an anhydro product at least on lengthy incubation during crystallisation. CONCLUSIONS: The structural simplification of MltA in Sps proteins is analogous to that of the resuscitation promoting factor domains of Actinobacteria, which are stripped down versions of lysozyme and soluble lytic transglycosylase proteins.

Structure of the stationary phase survival protein YuiC from B.subtilis.,Quay DH, Cole AR, Cryar A, Thalassinos K, Williams MA, Bhakta S, Keep NH BMC Struct Biol. 2015 Jul 11;15:12. doi: 10.1186/s12900-015-0039-z. PMID:26163297<ref>PMID:26163297</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wlk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Bhakta S]]
[[Category: Cole AR]]
[[Category: Cryar A]]
[[Category: Keep NH]]
[[Category: Quay DHX]]
[[Category: Thalassinos K]]
[[Category: Williams MA]]