View source for 4wwb


==High-resolution structure of the Ni-bound form of the Y135F mutant of C. metallidurans CnrXs==
<StructureSection load='4wwb' size='340' side='right'caption='[[4wwb]], [[Resolution|resolution]] 1.11&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wwb]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WWB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WWB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.11&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=FMT:FORMIC+ACID'>FMT</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wwb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wwb OCA], [https://pdbe.org/4wwb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wwb RCSB], [https://www.ebi.ac.uk/pdbsum/4wwb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wwb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CNRR_CUPMC CNRR_CUPMC] CnrH alone is able to activate cnr expression, while both CnrY and CrnR (CnrX) are needed for nickel induction of CnrH (PubMed:10671463). Has been suggested (PubMed:10671464) to bind nickel.<ref>PMID:10671463</ref> <ref>PMID:10671464</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Resistance to high concentration of nickel ions is mediated in Cupriavidus metallidurans by the CnrCBA transenvelope efflux complex. Expression of the cnrCBA genes is regulated by the transmembrane signal transduction complex CnrYXH. Together, the metal sensor CnrX and the transmembrane antisigma factor CnrY control the availability of the extracytoplasmic function sigma factor CnrH. Release of CnrH from sequestration by CnrY at the cytoplasmic side of the membrane depends essentially on the binding of the agonist metal ion Ni(ii) to the periplasmic metal sensor domain of CnrX. CnrH availability leads to transcription initiation at the promoters cnrYp and cnrCp and to the expression of the genes in the cnrYXHCBA nickel resistance determinant. The first steps of signal propagation by CnrX rely on subtle metal-dependent allosteric modifications. To study the nickel-mediated triggering process by CnrX, we have altered selected residues, F66, M123, and Y135, and explored the physiological consequences of these changes with respect to metal resistance, expression of a cnrCBA-lacZ reporter fusion and protein production. M123C- and Y135F-CnrXs have been further characterized in vitro by metal affinity measurements and crystallographic structure analysis. Atomic-resolution structures of metal-bound M123C- and Y135F-CnrXs showed that Ni(ii) binds two of the three canonical conformations identified and that Ni(ii) sensing likely proceeds by conformation selection.

Response of CnrX from Cupriavidus metallidurans CH34 to nickel binding.,Maillard AP, Kunnemann S, Grosse C, Volbeda A, Schleuder G, Petit-Hartlein I, de Rosny E, Nies DH, Coves J Metallomics. 2015 Jan 28. PMID:25628016<ref>PMID:25628016</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wwb" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cupriavidus metallidurans CH34]]
[[Category: Large Structures]]
[[Category: Coves J]]
[[Category: Grosse C]]
[[Category: Kuennemann S]]
[[Category: Maillard AP]]
[[Category: Nies DH]]
[[Category: Petit-Haertlein I]]
[[Category: Schleuder G]]
[[Category: Volbeda A]]
[[Category: De Rosny E]]