View source for 4wyv


==Crystal Structure of Human Translin in Open Conformation==
<StructureSection load='4wyv' size='340' side='right'caption='[[4wyv]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4wyv]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4jhf 4jhf]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WYV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4WYV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4wyv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wyv OCA], [https://pdbe.org/4wyv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4wyv RCSB], [https://www.ebi.ac.uk/pdbsum/4wyv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4wyv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/TSN_HUMAN TSN_HUMAN] DNA-binding protein that specifically recognizes consensus sequences at the breakpoint junctions in chromosomal translocations, mostly involving immunoglobulin (Ig)/T-cell receptor gene segments. Seems to recognize single-stranded DNA ends generated by staggered breaks occurring at recombination hot spots.<ref>PMID:9111049</ref> <ref>PMID:21552258</ref>   Exhibits both single-stranded and double-stranded endoribonuclease activity. May act as an activator of RNA-induced silencing complex (RISC) by facilitating endonucleolytic cleavage of the siRNA passenger strand.<ref>PMID:9111049</ref> <ref>PMID:21552258</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The single-stranded DNA (ssDNA)/RNA binding protein translin was suggested to be involved in chromosomal translocations, telomere metabolism, and mRNA transport and translation. Oligonucleotide binding surfaces map within a closed cavity of translin octameric barrels, raising the question as to how DNA/RNA gain access to this inner cavity, particularly given that, to date, none of the barrel structures reported hint to an entryway. Here, we argue against a mechanism by which translin octamers may "dissociate and reassemble" upon RNA binding and report a novel "open"-barrel structure of human translin revealing a feasible DNA/RNA entryway into the cavity. Additionally, we report that translin not only is confined to binding of ssDNA oligonucleotides, or single-stranded extensions of double-stranded DNA (dsDNA), but also can bind single-stranded sequences internally embedded in dsDNA molecules.

A Novel Open-Barrel Structure of Octameric Translin Reveals a Potential RNA Entryway.,Eliahoo E, Marx A, Manor H, Alian A J Mol Biol. 2014 Nov 26. pii: S0022-2836(14)00609-3. doi:, 10.1016/j.jmb.2014.11.013. PMID:25433126<ref>PMID:25433126</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4wyv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Alian A]]
[[Category: Dvir H]]
[[Category: Eliahoo E]]