View source for 4x4k


==Structure of laccase from Botrytis aclada with full copper content==
<StructureSection load='4x4k' size='340' side='right'caption='[[4x4k]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4x4k]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Botrytis_aclada Botrytis aclada]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4X4K OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4X4K FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4x4k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4x4k OCA], [https://pdbe.org/4x4k PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4x4k RCSB], [https://www.ebi.ac.uk/pdbsum/4x4k PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4x4k ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H8ZRU2_9HELO H8ZRU2_9HELO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Laccases belong to the class of multicopper oxidases catalyzing the oxidation of phenols accompanied by the reduction of molecular oxygen to water without the formation of hydrogen peroxide. The activity of laccases depends on the number of Cu atoms per enzyme molecule. The structure of type 2 copper-depleted laccase from Botrytis aclada has been solved previously. With the aim of obtaining the structure of the native form of the enzyme, crystals of the depleted laccase were soaked in Cu(+)- and Cu(2+)-containing solutions. Copper ions were found to be incorporated into the active site only when Cu(+) was used. A comparative analysis of the native and depleted forms of the enzymes was performed.

Incorporation of copper ions into crystals of T2 copper-depleted laccase from Botrytis aclada.,Osipov EM, Polyakov KM, Tikhonova TV, Kittl R, Dorovatovskii PV, Shleev SV, Popov VO, Ludwig R Acta Crystallogr F Struct Biol Commun. 2015 Dec 1;71(Pt 12):1465-9. doi:, 10.1107/S2053230X1502052X. Epub 2015 Nov 18. PMID:26625287<ref>PMID:26625287</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4x4k" style="background-color:#fffaf0;"></div>

==See Also==
*[[Laccase 3D structures|Laccase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Botrytis aclada]]
[[Category: Large Structures]]
[[Category: Kittl R]]
[[Category: Ludwig R]]
[[Category: Osipov EM]]
[[Category: Polyakov KM]]
[[Category: Popov VO]]
[[Category: Shleev SV]]
[[Category: Tikhonova TV]]