View source for 4xdz


==Holo structure of ketol-acid reductoisomerase from Ignisphaera aggregans==
<StructureSection load='4xdz' size='340' side='right'caption='[[4xdz]], [[Resolution|resolution]] 1.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xdz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ignisphaera_aggregans_DSM_17230 Ignisphaera aggregans DSM 17230]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XDZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XDZ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=40E:OXO(PROPAN-2-YLAMINO)ACETIC+ACID'>40E</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NDP:NADPH+DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xdz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xdz OCA], [https://pdbe.org/4xdz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xdz RCSB], [https://www.ebi.ac.uk/pdbsum/4xdz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xdz ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ILVC_IGNAA ILVC_IGNAA] Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH or NADH to yield (R)-2,3-dihydroxy-isovalerate.<ref>PMID:25172159</ref> <ref>PMID:26644020</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although most sequenced members of the industrially important ketol-acid reductoisomerase (KARI) family are Class I enzymes, structural studies to date have focused primarily on the Class II KARIs, which arose through domain duplication. Here, we present five new crystal structures of Class I KARIs. These include the first structure of a KARI with a 6-residue beta2alphaB (cofactor specificity determining) loop and an NADPH phosphate binding geometry distinct from that of the 7- and 12-residue loops. We also present the first structures of naturally occurring KARIs that utilize NADH as cofactor. These results show insertions in the specificity loops that confounded previous attempts to classify them according to loop length. Lastly, we explore the conformational changes that occur in Class I KARIs upon binding of cofactor and metal ions. The Class I KARI structures indicate that the active sites close upon binding NAD(P)H, similar to what is observed in the Class II KARIs of rice and spinach and different from the opening of the active site observed in the Class II KARI of E. coli. This conformational change involves a decrease in the bending of the helix that runs between the domains and a rearrangement of the nicotinamide binding site.

Cofactor specificity motifs and the induced fit mechanism in Class I ketol-acid reductoisomerases.,Cahn JK, Brinkmann-Chen S, Spatzal T, Wiig JA, Buller AR, Einsle O, Hu Y, Ribbe MW, Arnold FH Biochem J. 2015 Apr 7. PMID:25849365<ref>PMID:25849365</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xdz" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ketol-acid reductoisomerase 3D structures|Ketol-acid reductoisomerase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Ignisphaera aggregans DSM 17230]]
[[Category: Large Structures]]
[[Category: Arnold FH]]
[[Category: Brinkmann-Chen S]]
[[Category: Cahn JKB]]