View source for 4xrw


==Crystal structure of the di-domain ARO/CYC BexL from the BE-7585A biosynthetic pathway==
<StructureSection load='4xrw' size='340' side='right'caption='[[4xrw]], [[Resolution|resolution]] 1.79&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4xrw]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Amycolatopsis_orientalis Amycolatopsis orientalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XRW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XRW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.79&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xrw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xrw OCA], [https://pdbe.org/4xrw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xrw RCSB], [https://www.ebi.ac.uk/pdbsum/4xrw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xrw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D7RFJ9_AMYOR D7RFJ9_AMYOR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Aromatic polyketides make up a large class of natural products with diverse bioactivity. During biosynthesis, linear poly-beta-ketone intermediates are regiospecifically cyclized, yielding molecules with defined cyclization patterns that are crucial for polyketide bioactivity. The aromatase/cyclases (ARO/CYCs) are responsible for regiospecific cyclization of bacterial polyketides. The two most common cyclization patterns are C7-C12 and C9-C14 cyclizations. We have previously characterized three monodomain ARO/CYCs: ZhuI, TcmN, and WhiE. The last remaining uncharacterized class of ARO/CYCs is the di-domain ARO/CYCs, which catalyze C7-C12 cyclization and/or aromatization. Di-domain ARO/CYCs can further be separated into two subclasses: "nonreducing" ARO/CYCs, which act on nonreduced poly-beta-ketones, and "reducing" ARO/CYCs, which act on cyclized C9 reduced poly-beta-ketones. For years, the functional role of each domain in cyclization and aromatization for di-domain ARO/CYCs has remained a mystery. Here we present what is to our knowledge the first structural and functional analysis, along with an in-depth comparison, of the nonreducing (StfQ) and reducing (BexL) di-domain ARO/CYCs. This work completes the structural and functional characterization of mono- and di-domain ARO/CYCs in bacterial type II polyketide synthases and lays the groundwork for engineered biosynthesis of new bioactive polyketides.

Structural and functional analysis of two di-domain aromatase/cyclases from type II polyketide synthases.,Caldara-Festin G, Jackson DR, Barajas JF, Valentic TR, Patel AB, Aguilar S, Nguyen M, Vo M, Khanna A, Sasaki E, Liu HW, Tsai SC Proc Natl Acad Sci U S A. 2015 Dec 2. pii: 201512976. PMID:26631750<ref>PMID:26631750</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4xrw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Amycolatopsis orientalis]]
[[Category: Large Structures]]
[[Category: Aguilar S]]
[[Category: Barajas JF]]
[[Category: Caldara-Festin GM]]
[[Category: Jackson DR]]
[[Category: Khanna A]]
[[Category: Liu H-W]]
[[Category: Nguyen M]]
[[Category: Patel A]]
[[Category: Sasaki E]]
[[Category: Tsai SC]]
[[Category: Valentic TR]]
[[Category: Vo M]]