View source for 4y8w


==Crystal Structure of Human Cytochrome P450 21A2 Progesterone Complex==
<StructureSection load='4y8w' size='340' side='right'caption='[[4y8w]], [[Resolution|resolution]] 2.64&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4y8w]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Y8W OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Y8W FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.64&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=STR:PROGESTERONE'>STR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4y8w FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4y8w OCA], [https://pdbe.org/4y8w PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4y8w RCSB], [https://www.ebi.ac.uk/pdbsum/4y8w PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4y8w ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/CP21A_HUMAN CP21A_HUMAN] Classic congenital adrenal hyperplasia due to 21-hydroxylase deficiency, salt wasting form;Classic congenital adrenal hyperplasia due to 21-hydroxylase deficiency, simple virilizing form. The disease is caused by variants affecting the gene represented in this entry.
== Function ==
[https://www.uniprot.org/uniprot/CP21A_HUMAN CP21A_HUMAN] A cytochrome P450 monooxygenase that plays a major role in adrenal steroidogenesis. Catalyzes the hydroxylation at C-21 of progesterone and 17alpha-hydroxyprogesterone to respectively form 11-deoxycorticosterone and 11-deoxycortisol, intermediate metabolites in the biosynthetic pathway of mineralocorticoids and glucocorticoids (PubMed:10602386, PubMed:16984992, PubMed:22014889, PubMed:25855791, PubMed:27721825). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase) (PubMed:25855791).<ref>PMID:10602386</ref> <ref>PMID:16984992</ref> <ref>PMID:22014889</ref> <ref>PMID:25855791</ref> <ref>PMID:27721825</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cytochrome P450 (P450) 21A2 is the major steroid 21-hydroxylase, and deficiency of this enzyme is involved in ~ 95% of cases of human congenital adrenal hyperplasia, a disorder of adrenal steroidogenesis. A structure of the bovine enzyme we previously published (Zhao, B. et al. (2012) J. Biol. Chem. 287, 10613-10622), containing two molecules of the substrate 17alpha-hydroxyprogesterone, has been used as a template for understanding genetic deficiencies. We have now obtained a crystal structure of human P450 21A2 in complex with progesterone, a substrate in adrenal 21-hydroxylation. Substrate binding and release were fast for human P450 21A2 with both substrates, and pre-steady-state kinetics showed a partial burst but only with progesterone as substrate and not 17alpha-hydroxyprogesterone. High intermolecular non-competitive kinetic deuterium isotope effects on both kcat and kcat/Km (5-11) were observed with both substrates, indicative of rate-limiting C-H bond cleavage and suggesting that the juxtaposition of the C21 carbon in the active site is critical for efficient oxidation. The estimated rate of binding of the substrate progesterone (kon 2.4 x 107 M-1 s-1) is only ~ 2-fold greater than the catalytic efficiency (kcat/Km 1.3 x 107 M-1 s-1) with this substrate, suggesting that the rate of substrate binding may also be partially rate-limiting. The structure of the human P450 21A2-substrate complex provides direct insight into mechanistic effects of genetic variants.

Human Cytochrome P450 21A2, the Major Steroid 21-Hydroxylase: Structure of the Enzyme-Progesterone Substrate Complex and Rate-Limiting C-H Bond Cleavage.,Pallan PS, Wang C, Lei L, Yoshimoto FK, Auchus RJ, Waterman MR, Guengerich FP, Egli M J Biol Chem. 2015 Apr 8. pii: jbc.M115.646307. PMID:25855791<ref>PMID:25855791</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4y8w" style="background-color:#fffaf0;"></div>

==See Also==
*[[Cytochrome P450 hydroxylase 3D structures|Cytochrome P450 hydroxylase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Egli M]]
[[Category: Lei L]]
[[Category: Pallan PS]]