View source for 4yak


==Ca. Korarchaeum cryptofilum dinucleotide forming Acetyl-coenzyme A synthetase 1 in complex with coenzyme A, acetyl-coenzyme A and with phosphorylated phosphohistidine segment (site I orientation)==
<StructureSection load='4yak' size='340' side='right'caption='[[4yak]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4yak]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Candidatus_Korarchaeum_cryptofilum_OPF8 Candidatus Korarchaeum cryptofilum OPF8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YAK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YAK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.464&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene>, <scene name='pdbligand=NEP:N1-PHOSPHONOHISTIDINE'>NEP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yak FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yak OCA], [https://pdbe.org/4yak PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yak RCSB], [https://www.ebi.ac.uk/pdbsum/4yak PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yak ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B1L3C9_KORCO B1L3C9_KORCO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The NDP-forming acyl-CoA synthetases (ACDs) catalyze the conversion of various CoA thioesters to the corresponding acids, conserving their chemical energy in form of ATP. The ACDs are the major energy-conserving enzymes in sugar and peptide fermentation of hyperthermophilic archaea. They are considered to be primordial enzymes of ATP synthesis in the early evolution of life. We present the first crystal structures, to our knowledge, of an ACD from the hyperthermophilic archaeon Candidatus Korachaeum cryptofilum. These structures reveal a unique arrangement of the ACD subunits alpha and beta within an alpha2beta2-heterotetrameric complex. This arrangement significantly differs from other members of the superfamily. To transmit an activated phosphoryl moiety from the Ac-CoA binding site (within the alpha subunit) to the NDP-binding site (within the beta subunit), a distance of 51 A has to be bridged. This transmission requires a larger rearrangement within the protein complex involving a 21-aa-long phosphohistidine-containing segment of the alpha subunit. Spatial restraints of the interaction of this segment with the beta subunit explain the necessity for a second highly conserved His residue within the beta subunit. The data support the proposed four-step reaction mechanism of ACDs, coupling acyl-CoA thioesters with ATP synthesis. Furthermore, the determined crystal structure of the complex with bound Ac-CoA allows first insight, to our knowledge, into the determinants for acyl-CoA substrate specificity. The composition and size of loops protruding into the binding pocket of acyl-CoA are determined by the individual arrangement of the characteristic subdomains.

Structure of NDP-forming Acetyl-CoA synthetase ACD1 reveals a large rearrangement for phosphoryl transfer.,Weisse RH, Faust A, Schmidt M, Schonheit P, Scheidig AJ Proc Natl Acad Sci U S A. 2016 Jan 19. pii: 201518614. PMID:26787904<ref>PMID:26787904</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 4yak" style="background-color:#fffaf0;"></div>

==See Also==
*[[Acetyl-CoA synthetase 3D structures|Acetyl-CoA synthetase 3D structures]]
== References ==
<references/>
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</StructureSection>
[[Category: Candidatus Korarchaeum cryptofilum OPF8]]
[[Category: Large Structures]]
[[Category: Scheidig AJ]]
[[Category: Weisse RH-J]]