View source for 4yeh


==Crystal structure of Mg2+ ion containing hemopexin fold from Kabuli chana (chickpea white) at 2.45A resolution reveals a structural basis of metal ion transport==
<StructureSection load='4yeh' size='340' side='right'caption='[[4yeh]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4yeh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cicer_arietinum Cicer arietinum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YEH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YEH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yeh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yeh OCA], [https://pdbe.org/4yeh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yeh RCSB], [https://www.ebi.ac.uk/pdbsum/4yeh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yeh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G1K3R9_CICAR G1K3R9_CICAR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Plant seeds contain a number of proteins which play important roles in the protection and the process of germination of seeds. We have isolated and purified a 25 kDa protein from Kabuli Chana (Cicer arietinum L., Chickpea-white, CW-25). The CW-25 protein was crystallized using 0.5 M magnesium acetate, 0.1 M sodium cacodylate and 20 % (w/v) polyethylene glycol 8000, pH 6.5. The crystals of CW-25 belonged to space group P3 with unit cell dimensions, a = b = 80.5 A, and c = 69.2 A. The structure of CW-25 was determined using molecular replacement method and refined to an R factor of 0.152. The buried surface area between two molecules was found to be approximately 653 A(2) indicating the formation of a weak homodimer. The polypeptide chain of CW-25 adopted a hemopexin-fold with four-bladed beta-propellers. The structure formed a central tunnel-like architecture. A magnesium ion was observed in the centre of the tunnel. It was located at distances varying between 2.3 and 2.7 A from five oxygen atoms of which four were backbone oxygen atoms belonging to residues, Asn7, Asp65, Asp121 and Asp174 while the fifth oxygen atom, O(delta1) was from the side chain of Asn7. The approximate length of the tunnel was 30 A. Furthermore, a series of carbonyl oxygen atoms were present along the internal face of the tunnel. The diameter of the tunnel varied from 4.6 to 6.2 A. The diameter and chemical environment of the tunnel clearly indicated that it might be used for the transport of various metal ions across the molecule.

Crystal Structure of Mg(2+) Containing Hemopexin-Fold Protein from Kabuli Chana (Chickpea-White, CW-25) at 2.45 A Resolution Reveals Its Metal Ion Transport Property.,Kumar S, Singh A, Yamini S, Dey S, Singh TP Protein J. 2015 Aug;34(4):284-90. doi: 10.1007/s10930-015-9624-z. PMID:26242869<ref>PMID:26242869</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4yeh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cicer arietinum]]
[[Category: Large Structures]]
[[Category: Bhushan A]]
[[Category: Dey S]]
[[Category: Kumar S]]
[[Category: Sharma S]]
[[Category: Singh A]]
[[Category: Singh TP]]
[[Category: Yamini S]]