View source for 4yo3


==Enteroaggregative Escherichia Coli TssA N-terminal fragment==
<StructureSection load='4yo3' size='340' side='right'caption='[[4yo3]], [[Resolution|resolution]] 3.37&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4yo3]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_042 Escherichia coli 042]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YO3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YO3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.37&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yo3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yo3 OCA], [https://pdbe.org/4yo3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yo3 RCSB], [https://www.ebi.ac.uk/pdbsum/4yo3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yo3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/D3GUX1_ECO44 D3GUX1_ECO44] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Contractile tails are composed of an inner tube wrapped by an outer sheath assembled in an extended, metastable conformation that stores mechanical energy necessary for its contraction. Contraction is used to propel the rigid inner tube towards target cells for DNA or toxin delivery. Although recent studies have revealed the structure of the contractile sheath of the type VI secretion system, the mechanisms by which its polymerization is controlled and coordinated with the assembly of the inner tube remain unknown. Here we show that the starfish-like TssA dodecameric complex interacts with tube and sheath components. Fluorescence microscopy experiments in enteroaggregative Escherichia coli reveal that TssA binds first to the type VI secretion system membrane core complex and then initiates tail polymerization. TssA remains at the tip of the growing structure and incorporates new tube and sheath blocks. On the basis of these results, we propose that TssA primes and coordinates tail tube and sheath biogenesis.

Priming and polymerization of a bacterial contractile tail structure.,Zoued A, Durand E, Brunet YR, Spinelli S, Douzi B, Guzzo M, Flaugnatti N, Legrand P, Journet L, Fronzes R, Mignot T, Cambillau C, Cascales E Nature. 2016 Mar 3;531(7592):59-63. doi: 10.1038/nature17182. Epub 2016 Feb 24. PMID:26909579<ref>PMID:26909579</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4yo3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli 042]]
[[Category: Large Structures]]
[[Category: Bebeacua C]]
[[Category: Brunet YR]]
[[Category: Cambillau C]]
[[Category: Cascales E]]
[[Category: Douzi B]]
[[Category: Durand E]]
[[Category: Journet L]]
[[Category: Legrand P]]
[[Category: Mignot T]]
[[Category: Spinelli S]]
[[Category: Zoued A]]