View source for 4yyc


==Crystal structure of trimethylamine methyltransferase from Sinorhizobium meliloti in complex with unknown ligand==
<StructureSection load='4yyc' size='340' side='right'caption='[[4yyc]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4yyc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sinorhizobium_meliloti_1021 Sinorhizobium meliloti 1021]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YYC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=UNL:UNKNOWN+LIGAND'>UNL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yyc OCA], [https://pdbe.org/4yyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yyc RCSB], [https://www.ebi.ac.uk/pdbsum/4yyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yyc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q92P20_RHIME Q92P20_RHIME] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The misidentification of a protein sample, or contamination of a sample with the wrong protein, may be a potential reason for the non-reproducibility of experiments. This problem may occur in the process of heterologous overexpression and purification of recombinant proteins, as well as purification of proteins from natural sources. If the contaminated or misidentified sample is used for crystallization, in many cases the problem may not be detected until structures are determined. In the case of functional studies, the problem may not be detected for years. Here several procedures that can be successfully used for the identification of crystallized protein contaminants, including: (i) a lattice parameter search against known structures, (ii) sequence or fold identification from partially built models, and (iii) molecular replacement with common contaminants as search templates have been presented. A list of common contaminant structures to be used as alternative search models was provided. These methods were used to identify four cases of purification and crystallization artifacts. This report provides troubleshooting pointers for researchers facing difficulties in phasing or model building.

Protein purification and crystallization artifacts: The tale usually not told.,Niedzialkowska E, Gasiorowska O, Handing KB, Majorek KA, Porebski PJ, Shabalin IG, Zasadzinska E, Cymborowski M, Minor W Protein Sci. 2016 Mar;25(3):720-33. doi: 10.1002/pro.2861. Epub 2016 Jan 26. PMID:26660914<ref>PMID:26660914</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4yyc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Sinorhizobium meliloti 1021]]
[[Category: Ahmed M]]
[[Category: Almo SC]]
[[Category: Bonanno J]]
[[Category: Cooper DR]]
[[Category: Cymborowski MT]]
[[Category: Gasiorowska OA]]
[[Category: Hammonds J]]
[[Category: Handing KB]]
[[Category: Minor W]]
[[Category: Niedzialkowska E]]
[[Category: Porebski PJ]]
[[Category: Seidel R]]
[[Category: Shabalin IG]]
[[Category: Stead M]]