View source for 4zce


==Crystal Structure of the dust mite allergen Der p 23 from Dermatophagoides pteronyssinus==
<StructureSection load='4zce' size='340' side='right'caption='[[4zce]], [[Resolution|resolution]] 1.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4zce]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dermatophagoides_pteronyssinus Dermatophagoides pteronyssinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZCE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZCE FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zce FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zce OCA], [https://pdbe.org/4zce PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zce RCSB], [https://www.ebi.ac.uk/pdbsum/4zce PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zce ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DEP23_DERPT DEP23_DERPT] Does not bind chitin in vitro.<ref>PMID:26602749</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
BACKGROUND: Der p 23 was recently identified in a European population as a major allergen and potentially a chitin binding protein. OBJECTIVE: This study sought to assess the importance of Der p 23 among other Dermatophagoides allergens in a North American population, and to determine the structure for functional characterization. METHODS: IgE binding to Der p 23, Der p 1, Der p 2, Der p 5, Der p 7, and Der p 8 was measured by ELISA. RNA-seq data from D. pteronyssinus were compared as estimates of allergen expression levels. The structure was analyzed by X-ray crystallography and NMR. RESULTS: Despite a high prevalence of Der p 23, (75% versus 87% and 79% for Der p 1 and Der p 2, respectively), the anti-Der p 23 IgE levels were relatively low. The patient response to the 6 allergens tested was variable (n=47), but on average anti-Der p 1 and anti-Der p 2 together accounted for 85% of the specific IgE. In terms of abundance, the RNA expression level of Der p 23 is the lowest of the major allergens, 30-fold less that Der p 1 and 7-fold less than Der p 2. The structure of Der p 23 is a small, globular protein stabilized by two disulfide bonds, which is structurally related to allergens such as Blo t 12 that contain carbohydrate binding domains that bind chitin. Functional assays failed to confirm chitin binding by Der p 23. CONCLUSIONS AND CLINICAL RELEVANCE: Der p 23 accounts for a small percentage of the IgE response to mite allergens, which is dominated by Der p 1 and Der p 2. The prevalence and amount of specific IgE to Der p 23 and Der p 2 are disproportionately high compared to abundance of other Dermatophagoides allergens. This article is protected by copyright. All rights reserved.

Serological, genomic, and structural analyses of the major mite allergen Der p 23.,Mueller GA, Randall TA, Glesner J, Pedersen LC, Perera L, Edwards LL, DeRose EF, Chapman MD, London RE, Pomes A Clin Exp Allergy. 2015 Nov 25. doi: 10.1111/cea.12680. PMID:26602749<ref>PMID:26602749</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4zce" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Dermatophagoides pteronyssinus]]
[[Category: Large Structures]]
[[Category: Chapman MD]]
[[Category: Edwards LL]]
[[Category: Glesner J]]
[[Category: London RE]]
[[Category: Mueller GA]]
[[Category: Pedersen LC]]
[[Category: Perera L]]
[[Category: Pomes A]]
[[Category: Randall TA]]