View source for 4zg1


==Crystal structure of a nanobody raised against KDM5B==
<StructureSection load='4zg1' size='340' side='right'caption='[[4zg1]], [[Resolution|resolution]] 1.85&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4zg1]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZG1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZG1 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zg1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zg1 OCA], [https://pdbe.org/4zg1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zg1 RCSB], [https://www.ebi.ac.uk/pdbsum/4zg1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zg1 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
The histone demethylase KDM5B is considered to be a promising target for anticancer therapy. Single-chain antibodies from llama (nanobodies) have been raised to aid in crystallization and structure determination of this enzyme. The antigen-binding properties of 15 of these nanobodies have been characterized. The crystal structure of one of these (NB17) has been determined to a resolution of 1.85 A. NB17 crystallizes in space group P4322 with six molecules in the asymmetric unit. The six molecules in the asymmetric unit pack as an entity with approximate D3 symmetry with interactions mediated by the CDR loops, which could act as a crystallization nucleus. NB17 does not bind to monomeric KDM5B residues 1-820, but is found to bind to aggregates formed after incubation at 310 K.

Structure and binding properties of a cameloid nanobody raised against KDM5B.,Wiuf A, Kristensen LH, Kristensen O, Dorosz J, Jensen J, Gajhede M Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1235-41. doi:, 10.1107/S2053230X1501537X. Epub 2015 Sep 23. PMID:26457512<ref>PMID:26457512</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Lama glama]]
[[Category: Large Structures]]
[[Category: Gajhede M]]
[[Category: Kristensen O]]
[[Category: Wiuf A]]