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==Crystal structure of BbKI, a disulfide-free plasma kallikrein inhibitor at 1.4 A resolution==
<StructureSection load='4zot' size='340' side='right'caption='[[4zot]], [[Resolution|resolution]] 1.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4zot]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZOT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZOT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.4&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zot FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zot OCA], [https://pdbe.org/4zot PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zot RCSB], [https://www.ebi.ac.uk/pdbsum/4zot PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zot ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BBKI_BAUBA BBKI_BAUBA] 
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== Publication Abstract from PubMed ==
A serine protease inhibitor from Bauhinia bauhinioides (BbKI) belongs to the Kunitz family of plant inhibitors, which are common in plant seeds. BbKI does not contain any disulfides, unlike most other members of this family. It is a potent inhibitor of plasma kallikrein, in addition to other serine proteases, and thus exhibits antithrombotic activity. A high-resolution crystal structure of recombinantly expressed BbKI was determined (at 1.4 A resolution) and was compared with the structures of other members of the family. Modeling of a complex of BbKI with plasma kallikrein indicates that changes in the local structure of the reactive loop that includes the specificity-determining Arg64 are necessary in order to explain the tight binding. An R64A mutant of BbKI was found to be a weaker inhibitor of plasma kallikrein, but was much more potent against plasmin, suggesting that this mutant may be useful for preventing the breakup of fibrin and maintaining clot stability, thus preventing excessive bleeding.

Structure of BbKI, a disulfide-free plasma kallikrein inhibitor.,Zhou D, Hansen D, Shabalin IG, Gustchina A, Vieira DF, de Brito MV, Araujo AP, Oliva ML, Wlodawer A Acta Crystallogr F Struct Biol Commun. 2015 Aug 1;71(Pt 8):1055-62. doi:, 10.1107/S2053230X15011127. Epub 2015 Jul 29. PMID:26249699<ref>PMID:26249699</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4zot" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bauhinia bauhinioides]]
[[Category: Large Structures]]
[[Category: Oliva MLV]]
[[Category: Shabalin IG]]
[[Category: Wlodawer A]]
[[Category: Zhou D]]