View source for 5afd


==Native structure of N-acetylneuramininate lyase (sialic acid aldolase) from Aliivibrio salmonicida==
<StructureSection load='5afd' size='340' side='right'caption='[[5afd]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5afd]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aliivibrio_salmonicida_LFI1238 Aliivibrio salmonicida LFI1238]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AFD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.65&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5afd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5afd OCA], [https://pdbe.org/5afd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5afd RCSB], [https://www.ebi.ac.uk/pdbsum/5afd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5afd ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B6EI04_ALISL B6EI04_ALISL] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
N-acetylneuraminate lyases (NALs) are enzymes that catalyze the reversible cleavage and synthesis of sialic acids. They are therefore commonly used for the production of these high-value sugars. This study presents the recombinant production, together with biochemical and structural data, of the NAL from the psychrophilic bacterium Aliivibrio salmonicida LFI1238 (AsNAL). Our characterization shows that AsNAL possesses high activity and stability at alkaline pH. We confirm that these properties allow for the use in a one-pot reaction at alkaline pH for the synthesis of N-acetylneuraminic acid (Neu5Ac, the most common sialic acid) from the inexpensive precursor N-acetylglucosamine. We also show that the enzyme has a cold active nature with an optimum temperature for Neu5Ac synthesis at 20 degrees C. The equilibrium constant for the reaction was calculated at different temperatures, and the formation of Neu5Ac acid is favored at low temperatures, making the cold active enzyme a well-suited candidate for use in such exothermic reactions. The specific activity is high compared to the homologue from Escherichia coli at three tested temperatures, and the enzyme shows a higher catalytic efficiency and turnover number for cleavage at 37 degrees C. Mutational studies reveal that amino acid residue Asn 168 is important for the high kcat. The crystal structure of AsNAL was solved to 1.65 A resolution and reveals a compact, tetrameric protein similar to other NAL structures. The data presented provides a framework to guide further optimization of its application in sialic acid production and opens the possibility for further design of the enzyme.

Features and structure of a cold active N-acetylneuraminate lyase.,Gurung MK, Altermark B, Helland R, Smalas AO, Raeder ILU PLoS One. 2019 Jun 11;14(6):e0217713. doi: 10.1371/journal.pone.0217713., eCollection 2019. PMID:31185017<ref>PMID:31185017</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5afd" style="background-color:#fffaf0;"></div>

==See Also==
*[[N-acetylneuraminate lyase 3D structures|N-acetylneuraminate lyase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Aliivibrio salmonicida LFI1238]]
[[Category: Large Structures]]
[[Category: Altermark B]]
[[Category: Gurung MK]]
[[Category: Helland R]]
[[Category: Rader ILU]]
[[Category: Smalas AO]]