View source for 5ahy


==Halorhodopsin from Halobacterium salinarum in a new rhombohedral crystal form==
<StructureSection load='5ahy' size='340' side='right'caption='[[5ahy]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5ahy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum Halobacterium salinarum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AHY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AHY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=RET:RETINAL'>RET</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ahy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ahy OCA], [https://pdbe.org/5ahy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ahy RCSB], [https://www.ebi.ac.uk/pdbsum/5ahy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ahy ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/BACH_HALS3 BACH_HALS3] Light-driven chloride pump.<ref>PMID:24409552</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Halorhodopsin from the halophilic archaeon Halobacterium salinarum is a membrane located light-driven chloride pump. Upon illumination Halorhodopsin undergoes a reversible photocycle initiated by the all-trans to 13-cis isomerization of the covalently bound retinal chromophore. The photocycle consists of several spectroscopically distinct intermediates. The structural basis of the chloride transport mechanism remains elusive, presumably because packing contacts have so far precluded protein conformational changes in the available crystals. With the intention to structurally characterize late photocycle intermediates by X-ray crystallography we crystallized Halorhodopsin in a new crystal form using the vesicle fusion method. In the new crystal form lateral contacts are mediated by helices A and G. Helices E and F that were suggested to perform large movements during the photocycle are almost unrestrained by packing contacts. This feature might permit the displacement of these helices without disrupting the crystal lattice. Therefore, this new crystal form might be an excellent system for the structural characterization of late Halorhodopsin photocycle intermediates by trapping or by time resolved experiments, especially at XFELs.

Structure of Halorhodopsin from Halobacterium salinarum in a new crystal form that imposes little restraint on the E-F loop.,Schreiner M, Schlesinger R, Heberle J, Niemann HH J Struct Biol. 2015 Apr 24. pii: S1047-8477(15)00082-9. doi:, 10.1016/j.jsb.2015.04.010. PMID:25916754<ref>PMID:25916754</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 5ahy" style="background-color:#fffaf0;"></div>

==See Also==
*[[Bacteriorhodopsin 3D structures|Bacteriorhodopsin 3D structures]]
*[[Rhodopsin 3D structures|Rhodopsin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Halobacterium salinarum]]
[[Category: Large Structures]]
[[Category: Heberle J]]
[[Category: Niemann HH]]
[[Category: Schlesinger R]]
[[Category: Schreiner M]]