View source for 5aj0


==Cryo electron microscopy of actively translating human polysomes (POST state).==
<SX load='5aj0' size='340' side='right' viewer='molstar' caption='[[5aj0]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5aj0]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5AJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5AJ0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5aj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5aj0 OCA], [https://pdbe.org/5aj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5aj0 RCSB], [https://www.ebi.ac.uk/pdbsum/5aj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5aj0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RL27_HUMAN RL27_HUMAN] 
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== Publication Abstract from PubMed ==
Macromolecular machines, such as the ribosome, undergo large-scale conformational changes during their functional cycles. Although their mode of action is often compared to that of mechanical machines, a crucial difference is that, at the molecular dimension, thermodynamic effects dominate functional cycles, with proteins fluctuating stochastically between functional states defined by energetic minima on an energy landscape. Here, we have used cryo-electron microscopy to image ex-vivo-derived human polysomes as a source of actively translating ribosomes. Multiparticle refinement and 3D variability analysis allowed us to visualize a variety of native translation intermediates. Significantly populated states include not only elongation cycle intermediates in pre- and post-translocational states, but also eEF1A-containing decoding and termination/recycling complexes. Focusing on the post-translocational state, we extended this assessment to the single-residue level, uncovering striking details of ribosome-ligand interactions and identifying both static and functionally important dynamic elements.

Structural snapshots of actively translating human ribosomes.,Behrmann E, Loerke J, Budkevich TV, Yamamoto K, Schmidt A, Penczek PA, Vos MR, Burger J, Mielke T, Scheerer P, Spahn CM Cell. 2015 May 7;161(4):845-57. doi: 10.1016/j.cell.2015.03.052. PMID:25957688<ref>PMID:25957688</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 5aj0" style="background-color:#fffaf0;"></div>

==See Also==
*[[3D sructureseceptor for activated protein kinase C 1|3D sructureseceptor for activated protein kinase C 1]]
== References ==
<references/>
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</SX>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Behrmann E]]
[[Category: Budkevich TV]]
[[Category: Burger J]]
[[Category: Loerke J]]
[[Category: Mielke T]]
[[Category: Penczek PA]]
[[Category: Scheerer P]]
[[Category: Schmidt A]]
[[Category: Spahn CMT]]
[[Category: Vos MR]]
[[Category: Yamamoto K]]